CAD Antibody

Western Blot: CAD Antibody [NB100-61614] - Whole cell lysate (50 ug) from HEK293T, K-562, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAD antibody NB100-61614 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunohistochemistry-Paraffin: CAD Antibody [NB100-61614] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CAD used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Immunoprecipitation: CAD Antibody [NB100-61614] - Detection of human CAD by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CAD antibody NB100-61614 (lot NB100-61614-3) used for IP at 6 ug per reaction. CAD was also immunoprecipitated by a previous lot of this antibody (lot NB100-61614-2) and another rabbit anti-CAD antibody. For blotting immunoprecipitated CAD, NB100-61614 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CAD Antibody Summary

• Description: Novus Biologicals Rabbit CAD Antibody (NB100-61614) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-CAD Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1850 and 2000 of human carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase using the numbering given in entry NP_004332.2 (GeneID 790).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CAD Antibody

• CAD protein
• CAD trifunctional protein
• carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, anddihydroorotase
• EC 2.1.3.2
• EC 3.5.2.3
• EC 6.3.5
• EC 6.3.5.5
• multifunctional protein CAD

Background

Carbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis (1). This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway (2). Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus (3). While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD (4-5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CAD Antibody (NB100-61614)(2)

Publications using NB100-61614

• Huang Y, Bell LN, Okamura J et al. Phospho-deltaNp63alpha/SREBF1 protein interactions: Bridging cell metabolism and cisplatin chemoresistance Cell Cycle 2012-09-05 [PMID: 22951905] (WB, Human)
• Morin A, Fritsch L, Mathieu JR et al. Identification of CAD as an androgen receptor interactant and an early marker of prostate tumor recurrence. FASEB J 2012-01-01 [PMID: 21982950]

Bioinformatics

• Gene Symbol: CAD
• Entrez:
  • 790(Human)
  • 69719(Mouse)
• Uniprot:
  • NP_004332.2(Human)