CACYBP Antibody

Western Blot: CACYBP Antibody [NBP1-49922] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-CacyBP antibody NBP1-49922 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. CacyBP was also immunoprecipitated by rabbit anti-CacyBP antibody NBP1-49921, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Immunohistochemistry: CACYBP Antibody [NBP1-49922] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- CacyBP used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

CACYBP Antibody Summary

• Description: Novus Biologicals Rabbit CACYBP Antibody (NBP1-49922) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 200 and 228 of human Calcyclin Binding Protein using the numbering given in entry NP_055227.1 (GeneID 27101).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CACYBP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2-5 mcg/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CACYBP Antibody

• CacyBP
• calcyclin binding protein
• GIG5
• hCacyBP
• MGC87971
• RP1-102G20.6
• S100A6-binding protein
• S100A6BPgrowth-inhibiting gene 5 protein
• Siah-interacting protein (SIP)
• Siah-interacting protein
• SIPcalcyclin-binding protein

Background

The protein encoded by this gene is a calcyclin binding protein. It may be involved in calcium-dependent ubiquitination and subsequent proteosomal degradation of target proteins. It probably serves as a molecular bridge in ubiquitin E3 complexes and participates in the ubiquitin-mediated degradation of beta-catenin. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CACYBP