CACNB4 Antibody

Images

 
Western Blot: CACNB4 Antibody [NB100-55414] - (0.2ug/ml) staining of Human Bone Marrow lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

CACNB4 Antibody Summary

Immunogen
Peptide with sequence C-DYPDSYQDTYKPH corresponding to internal region (near C-Terminus) according to NP_000717.2, NP_001005746.1.
Epitope
DYPDSYQDTYKPH
Specificity
This antibody is expected to recognise all three reported isoforms (NP_001005747.1; NP_000717.2; NP_001005746.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CACNB4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 - 0.6 ug/ml
  • Peptide ELISA Detection limit 1:4000
Application Notes
WB: Approx. 55-60 kDa band observed in human bone marrow lysates (calculated MW of 54.7 kDa band according to NP_001005747.1 and 58.2 kDa band according to NP_000717.2).
Positive Control
CACNB4 Lysate (NBL1-08630)
Publications
Read Publication using NB100-55414.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CACNB4 Antibody

  • calcium channel beta-4 subunit
  • calcium channel, voltage-dependent, beta 4 subunit
  • EJM
  • EJM4
  • EJM6
  • voltage-dependent L-type calcium channel subunit beta-4

Background

This gene encodes a member of the beta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. The protein described in this record plays an important role in calcium channel function by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Certain mutations in this gene have been associated with idiopathic generalized epilepsy (IGE) and juvenile myoclonic epilepsy (JME). Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CACNB4 Antibody (NB100-55414)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CACNB4
Entrez
Uniprot