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CACNB2 Antibody (S8b-1) [Alexa Fluor® 647]

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clone
S8b-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 647

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP1-47607AF405
Alexa Fluor 488
NBP1-47607AF488
Alexa Fluor 700
NBP1-47607AF700
Biotin
NBP1-47607B
DyLight 350
NBP1-47607UV
DyLight 405
NBP1-47607V
DyLight 488
NBP1-47607G
DyLight 550
NBP1-47607R
DyLight 650
NBP1-47607C
DyLight 680
NBP1-47607FR
DyLight 755
NBP1-47607IR
FITC
NBP1-47607F
HRP
NBP1-47607H
Janelia Fluor 549
NBP1-47607JF549
Janelia Fluor 646
NBP1-47607JF646
Unconjugated
NBP1-47607
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CACNB2 Antibody (S8b-1) [Alexa Fluor® 647] Summary

Immunogen
Synthetic peptide amino acids 189-205 of rat CavBeta2
Localization
Cell Membrane, Sarcolemma
Specificity
Detects approx 78 kDa. No cross reactivity against CavB1, CavB3, CavB4.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CACNB2
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Alternate Names for CACNB2 Antibody (S8b-1) [Alexa Fluor® 647]

  • CAB2
  • CACNLB2
  • Calcium channel voltage-dependent subunit beta 2
  • calcium channel, voltage-dependent, beta 2 subunit
  • CAVB2
  • FLJ23743
  • Lambert-Eaton myasthenic syndrome antigen B
  • myasthenic (Lambert-Eaton) syndrome antigen B
  • MYSB
  • voltage-dependent L-type calcium channel subunit beta-2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Recombinant Human LIGHT/TNFSF14 (Catalog # 664-LI/CF) stimulates cell proliferation in HUVEC human umbilical vein endothelial cells. The ED50 is 1-4 ng/mL.
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Immunocytochemistry/Immunofluorescence: Cav1.2 Antibody [NBP2-76914] - ICC staining CACNA1C in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry: Cav1.2 Antibody [NBP2-76914] - Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CACNA1C antibody. Counter stained with hematoxylin.
Flow Cytometry: Cav1.2 Antibody [NBP2-76914] - Flow cytometric analysis of SKOV-3 cells with CACNA1C antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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NPDC‑1 was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Sheep Anti-Human NPDC‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5489) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and synaptic vesicles of neuronal processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Immunocytochemistry/Immunofluorescence: TRNT1 Antibody [NBP1-86589] - Staining of human cell line A-431 shows positivity in mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TRNT1 Antibody [NBP1-86589] - Staining of human stomach shows moderate cytoplasmic and nuclear positivity in glandular cells.
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Immunocytochemistry/Immunofluorescence: integrin beta 4 binding protein Antibody [NBP2-13954] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: integrin beta 4 binding protein Antibody [NBP2-13954] - Staining of human skeletal muscle shows low expression as expected.
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I/CA1 Antibody
NBP1-88191
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Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: Carbonic Anhydrase I/CA1 Antibody [NBP1-88191] - Analysis in human rectum and pancreas tissues. Corresponding CA1 RNA-seq data are presented for the same tissues.
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Immunocytochemistry/Immunofluorescence: Nav1.5 Antibody (4G8-1G7) [NB110-61010] - Figure A: Plasma membrane localisation of Nav1.5 identified by 4G8:1G7 detected by anti-mouse Alexa Fluor-488 conjugated antibody; Figure B, DAP1 co-staining of cell nuclei
Flow Cytometry: Nav1.5 Antibody (4G8:1G7) [NB110-61010] - An intracellular stain was performed on U2OS cells with Nav1.5 Antibody [4G8:1G7] NB110-61010PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
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CACNB1 Antibody
NBP2-76915
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Immunohistochemistry-Paraffin: CACNB1 Antibody [NBP2-76915] - Analysis of mouse brain tissue using anti-CACNB1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: CACNB1 Antibody [NBP2-76915] - Analysis of rat heart tissue using anti-CACNB1 antibody. Counter stained with hematoxylin.
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(20A)
NB120-2864
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[Unconjugated]
1129-ER
1 μg/lane of Recombinant Human ErbB2 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 125-130 kDa and  220-250 kDa, respectively.
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Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.
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CACNB2 Antibody
(S8b-1) [Alexa Fluor& ...
NBP1-47607AF647
CACNB2 Antibody (S8b-1) [Alexa Fluor® 647]
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P

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Product General Protocols

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FAQs for CACNB2 Antibody (NBP1-47607AF647) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP1-47607AF405
Alexa Fluor 488 NBP1-47607AF488
Alexa Fluor 647 NBP1-47607AF647
Alexa Fluor 700 NBP1-47607AF700
Biotin NBP1-47607B
DyLight 350 NBP1-47607UV
DyLight 405 NBP1-47607V
DyLight 488 NBP1-47607G
DyLight 550 NBP1-47607R
DyLight 650 NBP1-47607C
DyLight 680 NBP1-47607FR
DyLight 755 NBP1-47607IR
FITC NBP1-47607F
HRP NBP1-47607H
Janelia Fluor 549 NBP1-47607JF549
Janelia Fluor 646 NBP1-47607JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol CACNB2
Entrez
Uniprot