SCN3B Antibody (S396-29) - BSA Free

Western Blot: SCN3B Antibody (S396-29) [NBP2-59318] - Western Blot analysis of Mouse Brain showing detection of ~40 kDa Nav Beta 3 protein using Mouse Anti-Nav Beta 3 Monoclonal Antibody, Clone S396-29 (NBP2-59318). Lane 1: MW Ladder. Lane 2: Mouse Brain. Load: 20 ug. Primary Antibody: Mouse Anti-Nav Beta 3 Monoclonal Antibody (NBP2-59318) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Predicted/Observed Size: ~40 kDa.

Immunocytochemistry/Immunofluorescence: SCN3B Antibody (S396-29) [NBP2-59318] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nav beta3 Monoclonal Antibody, Clone S396-29 (NBP2-59318). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Nav beta3 Monoclonal Antibody (NBP2-59318) at 1:200 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Nav beta3 Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: SCN3B Antibody (S396-29) [NBP2-59318] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Nav beta 3 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane, Nucleus. Magnification: 60X.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ICC/IF
• Clone: S396-29
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

SCN3B Antibody (S396-29) - BSA Free Summary

• Description: Novus Biologicals Mouse SCN3B Antibody (S396-29) - BSA Free (NBP2-59318) is a monoclonal antibody validated for use in WB, ICC/IF and Simple Western. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Fusion protein amino acids 1-215 (full-length) of rat NavBeta3
• Localization: Membrane
• Specificity: Detects 40kDa. Does not cross-reat with NavBeta1, Navbeta2, or Navbeta4.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SCN3B
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Simple Western
  • Western Blot 1:1000
• Application Notes: This SCN3B Antibody (S396-29) has been validated for Simple Western from a verified customer review.
  See Simple Western Antibody Database for Simple Western validation: separated by Size

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.1% Sodium Azide
• Purity: Protein G purified

Alternate Names for SCN3B Antibody (S396-29) - BSA Free

• HSA243396
• KIAA1158
• SCNB3
• sodium channel subunit beta-3
• sodium channel, voltage-gated, type III, beta
• voltage-gated sodium channel beta-3 subunit

Background

Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit and one or more regulatory beta subunits. They are responsible for the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel beta subunit gene family, and influences the inactivation kinetics of the sodium channel. Two alternatively spliced variants, encoding the same protein, have been identified. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for SCN3B Antibody (NBP2-59318) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: auriane ernault Simple Western Rat 02/17/2021

Summary

• Application: Simple Western
• Sample Tested: primary neonatal ventricular cardiomyocytes
• Species: Rat
• Lot: 150325

Comments

• Comments: Antibody used at a dilution of 1:50. Protein concentration of 0.8ug/uL. ***Bio-Techne Response: This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.***

Bioinformatics

• Gene Symbol: SCN3B