Human CA15-3 ELISA Kit (Colorimetric)

Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum
• Standard Curve Range: 15 U/mL - 250 U/mL
• Sensitivity: 3.8 U/mL

Human CA15-3 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CA15-3. No significant cross-reactivity or interference between human CA15-3 and analogues was observed.
• Standard Curve Range: 15 U/mL - 250 U/mL
• Sensitivity: 3.8 U/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 20
• Intra-Assay: %CV < 15
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: MUC1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human CA15-3 ELISA Kit (Colorimetric)

• ADMCKD1
• ADMCKD2
• ADTKD2
• Breast carcinoma-associated antigen DF3
• CA153
• CA15-3
• Cancer antigen 15-3
• Carcinoma-associated mucin
• CD227
• EMA
• episialin
• H23 antigen
• H23AG
• KL-6
• Krebs von den Lungen-6
• MAM6
• MCD
• MCKD
• MCKD1
• MUC1
• MUC-1
• MUC-1/SEC
• MUC-1/X
• MUC1/ZD
• mucin 1, transmembrane
• Peanut-reactive urinary mucin
• PEM
• PEMT
• Polymorphic epithelial mucin
• PUM
• tumor associated epithelial mucin
• Tumor-associated epithelial membrane antigen

Background

This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for CA15-3 ELISA Kit (NBP2-59962)(1)

Publication using NBP2-59962

• Sahajal Dhooria, Siddhant Arora, Shivani Chaudhary, Inderpaul Singh Sehgal, Nidhi Prabhakar, Nasim Mohammad, Riya Sharma, Prabir Das, Yashwant Kumar, Mandeep Garg, Goverdhan Dutt Puri, Ashish Bhalla, Valliappan Muthu, Kuruswamy Thurai Prasad, Ritesh Agarwal, Ashutosh Nath Aggarwal Risk factors for clinically significant diffuse parenchymal lung abnormalities persisting after severe COVID-19 pneumonia. The Indian journal of medical research 2023-11-14 [PMID: 37322633]

FAQs for CA15-3 ELISA Kit (NBP2-59962). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: MUC1