Human CA125/MUC16 ELISA Kit (Colorimetric)

Human CA125/MUC16 ELISA Kit (Colorimetric) [NBP2-59961] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum and plasma

Human CA125/MUC16 ELISA Kit (Colorimetric) Summary

• Description: Assay Length: 1-5 hours
• Specificity: This assay has high sensitivity and excellent specificity for detection of human CA125. No significant cross-reactivity or interference between human CA125 and analogues was observed.
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 20
• Intra-Assay: %CV < 15
• Sample Volume: 50 - 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: MUC16

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human CA125/MUC16 ELISA Kit (Colorimetric)

• CA125 ovarian cancer antigen
• CA125
• CA-125
• CA125MUC-16
• FLJ14303
• MUC16
• mucin 16, cell surface associated
• mucin-16
• Ovarian cancer-related tumor marker CA125
• Ovarian carcinoma antigen CA125

Background

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CA125 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for CA125. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CA125 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: MUC16
• Uniprot:
  • Q8WXI7()