C5L2/GPR77 Antibody

Western Blot: C5L2/GPR77 Antibody [NB100-56497] - Analysis of GPR77 in human testis cell lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 0.5 ug/ml.

Immunohistochemistry-Paraffin: C5L2/GPR77 Antibody [NB100-56497] - Analysis of human liver using NB100-56497 at 10 ug/ml.

Product Discontinued

C5L2/GPR77 Antibody Summary

• Immunogen: A portion of amino acids 1-50 of human GPR77 was used as the immunogen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: C5AR2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:20-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 0.5-2 ug/ml
• Application Notes: Immunocytochemistry/Immunofluorescence: See Bamberg et al (2010) for details.
• Control:

  Human Testis Whole Tissue Lysate (Adult Whole Normal)
  C5L2/GPR77 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for C5L2/GPR77 Antibody

• C5a anaphylatoxin chemotactic receptor C5L2
• C5L2
• C5L2G-protein coupled receptor 77
• G protein-coupled receptor 77
• G protein-coupled receptor C5L2
• GPF77
• GPR77

Background

Official Gene Symbol: GPR77 Gen Bank Accession Number: NP_060955 Gene ID: 27202 (human) Gene Map Locus: 19q13.33 (human) C5L2 is 337A.A orphan member that belongs to anaphylatoxin receptor family. It is a seven transmembrane G-protein-coupled receptor consisting of an N-terminal N-glycosylation site, several conserved cysteines and several potential C-terminal phosphorylation sites. It binds to C5a and C5a-des-Arg and serves as a highly regulated scavenger receptor with a negative modulatory influence on C5aR-mediated inflammation. Northern Blot analysis suggests it is co- expressed along with C5aR in different tissues and cells including neutrophils/macrophages, mast cells, dendritic cells, brain, lung, heart, kidney, liver, ovary and testis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for C5L2/GPR77 Antibody (NB100-56497)(3)

Publications using NB100-56497

• Werner U, Kunstner A, Drenckhan M et al. Linking Complement C3 and B Cells in Nasal Polyposis J Immunol Res 2020-07-08 [PMID: 32724828] (WB, Human)
• Croker DE, Halai R, Kaeslin G et al. C5a2 can modulate ERk1/2 signaling in macrophages via heteromer formation with C5a1 and beta-arrestin recruitment. Immunol. Cell Biol. 2014-04-29 [PMID: 24777312] (Human)
  
  Details:
  Monocyte-derived macrophages, Fig 5C. The pAb was used at 5 ug/ml in an attempt to block ERk1/2 activation.
• Bamberg CE, Mackay CR, Lee H et al. The C5a receptor (C5aR) C5L2 is a modulator of C5aR-mediated signal transduction. J Biol Chem. 2010-03-05 [PMID: 20044484] (ICC/IF, Human)
  
  Details:
  C5L2 (IMG-5888A): stained human polymorphonuclear leukocytes (PMN), IF/ICC (images analyzed by confocal microscopy), Fig 7.

Bioinformatics

• Gene Symbol: C5AR2
• Entrez:
  • 27202(Human)
• Uniprot:
  • Q9P296()