Human C4 binding protein A ELISA Kit (Colorimetric)

ELISA: Human C4 binding protein A ELISA Kit (Colorimetric) [NBP2-60550] - Standard curve.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, Plasma, Cell Culture, Urine, Saliva, Milk, CSF
• Standard Curve Range: 0.469 - 30 ng/mL (example only; lot dependent)
• Sensitivity: 0.9 ng/mL (example only; lot dependent)

Human C4 binding protein A ELISA Kit (Colorimetric) Summary

• Description: This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.
• Standard Curve Range: 0.469 - 30 ng/mL (example only; lot dependent)
• Sensitivity: 0.9 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 7.10 (example only; lot dependent)
• Intra-Assay: %CV < 5.00 (example only; lot dependent)
• Spike Recovery: 97.00% (example only; lot dependent)
• Kit Type: ELISA Kit (Colorimetric)
• Gene: C4BPA

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human C4 binding protein A ELISA Kit (Colorimetric)

• C4b binding protein, alpha chain
• C4b-binding protein alpha chain
• C4BP
• complement component 4 binding protein, alpha chain
• complement component 4 binding protein, alpha
• complement component 4-binding protein, alpha
• Proline-rich protein
• PRP

Background

C4 binding protein A encodes a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. Along with a single, unique beta-chain, seven identical alpha-chains encoded by this gene assemble into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. The genes encoding both alpha and beta chains are located adjacent to each other on human chromosome 1 in the regulator of complement activation gene cluster. Two pseudogenes of this gene are also found in the cluster. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for C4 binding protein A ELISA Kit (NBP2-60550)(1)

Publication using NBP2-60550

• Soares Martins T, MarCalo R, da Cruz E Silva CB et al. Novel Exosome Biomarker Candidates for Alzheimer's Disease Unravelled Through Mass Spectrometry Analysis Molecular neurobiology 2022-02-25 [PMID: 35212939] (ELISA)

Review for C4 binding protein A ELISA Kit (NBP2-60550) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Tânia Martins 04/05/2022

Summary

• Sample Tested: human serum exosomes
• Lot: 092112016 and 022111802

Comments

• Comments: The ELISA kit was rapid and easy to perform. I could measure C4BPalpha using little amounts of samples.

FAQs for C4 binding protein A ELISA Kit (NBP2-60550). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: C4BPA