Preparation Method |
Knockout achieved by using CRISPR/Cas9,195 bp insertion in exon1 and Insertion of the selection cassette in exon1 |
Gene | JUN |
Dilutions |
|
Application Notes | You will receive 1 vial (100ug) of knockout cell lysate and 1 vial (100ug) of Parental cell lysate. Lysate can be diluted with 1X SDS sample buffer and will be stable at -20 degrees C for 12 months. Minimize freeze-thaw cycles. |
Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
Buffer | 0.1 mg cell homogenate lyophilized in RIPA buffer made with double-knockout cell lines. |
Concentration | LYOPH |
Reconstitution Instructions | To use as WB negative control, spin down briefly and resuspend in 100 uL 1xSDS sample buffer (2% SDS, 60 mM Tris-HCl pH 6.8, 10% Glycerol, 0.02% Bromophenol blue, 60 mM beta-mercaptoethanol). Boil the lysate for 3 - 5 minutes before loading it onto gel. |
Research Areas for c-jun Lysate (NBP2-65447)Find related products by research area.
|
Read full blog post. |
Estrogen Related Receptors Play Roles in Cancer and Neurodegeneration By Eric NeeleyEstrogen receptors come in the form of two distinct forms, ER alpha and ER beta. These nuclear receptors are predominantly activated by the hormone 17-beta-estradiol to control transcription of genes throughout the immune, nervous, car... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
Gene Symbol | JUN |