c-Fos Antibody

Images

 
Western Blot: c-Fos Antibody [NB100-91772] - Analysis of Fos (F3) pAb at 1:500 dilution Lane1:Hela whole cell lysate (40 ug) Lane2:PC12 whole cell lysate(40 ug) Lane3:NIH-3T3 whole cell lysate(40 ug)
Immunohistochemistry-Paraffin: c-Fos Antibody [NB100-91772] - Paraffin-embedded human breast carcinoma tissue.
Western Blot: c-Fos Antibody [NB100-91772] - Lane1:Hela whole cell lysate. Lane2:Mouse brain tissue lysate. Lane3: Mouse heart tissue lysate, Lane5:Rat kidney tissue lysate. Lane6:Rat brain tissue lysate.
Western Blot: c-Fos Antibody [NB100-91772] - Extracts from HepG2 cells.
Immunohistochemistry-Paraffin: c-Fos Antibody [NB100-91772] - Human breast carcinoma tissue.

Product Details

Summary
Product Discontinued
View other related c-Fos Primary Antibodies

Order Details


    • Catalog Number
      NB100-91772
    • Availability
      Product Discontinued

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c-Fos Antibody Summary

Immunogen
Synthetic peptide, corresponding to amino acids 1-50 of Human c-Fos.
Clonality
Polyclonal
Host
Rabbit
Gene
FOS
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Application Notes
Western blot (WB) analysis of Fos (F3) pAb in extracts from HepG2 cells.
Immunohistochemistry (IHC) analyzes of Fos (F3) pAb in paraffin-embedded human breast carcinoma tissue.
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
c-Fos Lysate (NBL1-10794)
Reviewed Applications
Read 1 Review rated 4
using
NB100-91772 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2)
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for c-Fos Antibody

  • activator protein 1
  • AP-1
  • cellular oncogene c-fos
  • Cellular oncogene fos
  • cFos
  • c-Fos
  • FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS)
  • FBJ murine osteosarcoma viral oncogene homolog
  • FOS
  • G0/G1 switch regulatory protein 7
  • G0S7
  • proto-oncogene c-Fos
  • v-fos FBJ murine osteosarcoma viral oncogene homolog

Background

The v-Fos oncogene was initially identified as the transforming gene of two independent murine osteosarcoma virus isolates and an avian nephroblastoma virus. The cellular homolog, c-Fos, encodes a nuclear phosphoprotein that is rapidly and transiently induced by a variety of agents and functions as a transcriptional regulator for several genes. In contrast to c-Jun proteins, which form homo- and heterodimers which bind to specific DNA TPA response elements (TREs), c-Fos proteins are only active as heterodimers with members of the Jun gene family. Murine Fos B encodes a nuclear protein of 338 amino acids which has 70% homology with c-Fos, exhibits similar kinetics of expression as c-Fos and forms heterodimers with both c-Jun and Jun B, which bind to TRE DNA response elements. Functional homologs of c-Fos and Fos B include Fra-1 and Fra-2 genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for c-Fos Antibody (NB100-91772) (0)

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Review for c-Fos Antibody (NB100-91772) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IHC.

Reviews using NB100-91772:
Filter by Applications
IHC
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC Mouse 12/28/2011
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Summary

ApplicationImmunohistochemistry
Sample TestedMouse
SpeciesMouse
CommentsGood antibody

Blocking

Blocking DetailsBlocking Buffer: 1% BSA

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:100, Primary Ab Incubation Time: 2 hours

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-rabbit HRP

Details

Detection NotesDetection Visualization Method: DAB

Comments

CommentsGood antibody

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for c-Fos Antibody (NB100-91772). (Showing 1 - 1 of 1 FAQ).

  1. I am looking for some c-fos antibodies that could work in IHC.
    • Here are a list of products which might be of interest to you: Product List. We have additional filters on the left which may help you narrow down based on host species, target species, clonality, etc. We will guarantee these to work for all stated species and applications listed on the datasheet.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for c-Fos Antibody (NB100-91772)

Discover related pathways, diseases and genes to c-Fos Antibody (NB100-91772). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

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By Eric NeeleyEstrogen receptors come in the form of two distinct forms, ER alpha and ER beta. These nuclear receptors are predominantly activated by the hormone 17-beta-estradiol to control transcription of genes throughout the immune, nervous, car...  Read full blog post.

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Calculators

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Recent Reviews

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1
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Anonymous
12/28/2011
Application: IHC
Species: Mouse

Bioinformatics

Gene Symbol FOS
Entrez
Uniprot