Butyrylcholinesterase/BCHE Overexpression Lysate (Native)

Images

 
Western Blot: Butyrylcholinesterase Overexpression Lysate (Adult Normal) [NBL1-07942] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Butyrylcholinesterase.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Butyrylcholinesterase/BCHE Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202198 and based on accession number NM_000055. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens butyrylcholinesterase (BCHE), mRNA.
Gene
BCHE

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot, with an expected band ~79 kDa. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
68.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Butyrylcholinesterase/BCHE

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Butyrylcholinesterase/BCHE Overexpression Lysate (Native)

  • Acylcholine acylhydrolase
  • BCHE
  • Butyrylcholine esterase
  • Butyrylcholinesterase
  • CHE1
  • CHE1cholinesterase
  • Choline esterase II
  • cholinesterase 1
  • E1
  • EC 3.1.1.8
  • Pseudocholinesterase

Background

Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for Butyrylcholinesterase/BCHE Lysate (NBL1-07942) (0)

There are no publications for Butyrylcholinesterase/BCHE Lysate (NBL1-07942).
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Butyrylcholinesterase/BCHE Lysate (NBL1-07942). (Showing 1 - 1 of 1 FAQ).

  1. How much Butyrylcholinesterase lysate[NBL1-07942] do you use in the Butyrylcholinesterase Lysate Images? (What amount of protein should I load for overexperssion lysates?)
    • The lysate WB images you inquired about show 10ug of transfected lysate and 10ug of empty vector control lysate being loaded per well. (We recommend loading 10ug of protein per lane of our overexpression lysates. The end user will need to determine their own optimal working amounts.)

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Bioinformatics

Gene Symbol BCHE
Entrez