Bub3 Antibody

Images

 
Western Blot: Bub3 Antibody [NBP1-33558] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BUB3 antibody diluted by 1:1000.
Immunohistochemistry-Paraffin: Bub3 Antibody [NBP1-33558] - Paraffin-embedded A549 Xenograft, using antibody at 1:100 dilution.
Western Blot: Bub3 Antibody [NBP1-33558] - Sample (30 ug of whole cell lysate) A: 293T B: H1299 10% SDS PAGE; antibody diluted at 1:1000.
Immunoprecipitation: Bub3 Antibody [NBP1-33558] - Immunoprecipitation of BUB3 protein from 293T whole cell extracts using 5 ug of BUB3 antibody [N1C1]. Western blot analysis was performed using BUB3 antibody [N1C1]. ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ch, RM, XpSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.32 mg/ml

Order Details

Bub3 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human BUB3. The exact sequence is proprietary.
Localization
Nucleus
Predicted Species
Mouse (100%), Rat (100%), Chicken (96%), Xenopus (92%), Rhesus Macaque (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
BUB3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (92%), Zebrafish (83%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.32 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Bub3 Antibody

  • BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog
  • BUB3 budding uninhibited by benzimidazoles 3 homolog
  • Bub3
  • BUB3L
  • budding uninhibited by benomyl
  • budding uninhibited by benzimidazoles 3 homolog (yeast)
  • hBUB3
  • mitotic checkpoint component
  • mitotic checkpoint protein BUB3

Background

The spindle checkpoint ensures the fidelity of chromosome segregation in mitosis and meiosis. In response to defects in the mitotic apparatus, it blocks the activity of the anaphase-promoting complex, a large ubiquitin ligase required for chromosome segregation. Recent studies indicate that the spindle checkpoint monitors both the attachment of chromosomes to the mitotic spindle and the tension across the sister chromatid generated by microtubules. Upon checkpoint activation, checkpoint protein complexes containing BubR1(Mad3), Bub3, Mad2 and Cdc20 directly bind to the anaphase-promoting complex and inhibit its ligase activity. Therefore, the checkpoint proteins form a complex intracellular signalling network to inhibit the anaphase-promoting complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BUB3
Entrez