BTN3A1/2/3 Antibody (849203) [Phycoerythrin]

Human peripheral blood lymphocytes were stained with Mouse Anti-Human BTN3A1/2/3 PE-conjugated Monoclonal Antibody (Catalog # FAB7136P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 849203
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

BTN3A1/2/3 Antibody (849203) [Phycoerythrin] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human CD277/BTN3A1
  Met1-Gly254
  Accession # O00481
• Specificity: Detects human BTN3A1, BTN3A2 and BTN3A3 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BTN3A1
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BTN3A1/2/3 Antibody (849203) [Phycoerythrin]

• BT3.1
• BTF5
• BTN3.1
• BTN3A1/2/3
• butyrophilin subfamily 3 member A1
• CD277
• dJ45P21.3 (butyrophilin, subfamily 3, member A1)

Background

Butyrophilin BTN3A1, also known as CD277, has high structural homology to the B7 superfamily of proteins and is expressed in various immune cells such as T and NK cells. BTN3A1 enhances TCR-induced cytokine production and cell proliferation. Early T-cell activation events such as TCR-induced cell signaling are increased upon BTN3A1 engagement. The BTN3A1 co-stimulatory pathway may be involved in the regulation of various cell-mediated immune responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BTN3A1/2/3 Antibody (FAB7136P)(1)

Publication using FAB7136P

• Mamedov, MR;Vedova, S;Freimer, JW;Sahu, AD;Ramesh, A;Arce, MM;Meringa, AD;Ota, M;Chen, PA;Hanspers, K;Nguyen, VQ;Takeshima, KA;Rios, AC;Pritchard, JK;Kuball, J;Sebestyen, Z;Adams, EJ;Marson, A; CRISPR screens decode cancer cell pathways that trigger ?? T cell detection Nature 2023-08-30 [PMID: 37648854] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: BTN3A1
• Uniprot:
  • O00481(Human)