BTLA/CD272 Antibody [PerCP (Peridinin-chlorophyll Protein Complex)]

Human whole blood lymphocytes were stained with Mouse Anti-Human CD19 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB4867F) and either (A) Goat Anti-Human BTLA PerCP-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB3354C) or (B) Normal Goat IgG PerCP Control (Catalog # IC108C). View our protocol for Staining Membrane-associated Proteins.

Product Discontinued

BTLA/CD272 Antibody [PerCP (Peridinin-chlorophyll Protein Complex)] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BTLA
  Ile25-Ser150
  Accession # AAP44003
• Specificity: Detects human BTLA in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BTLA
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BTLA/CD272 Antibody [PerCP (Peridinin-chlorophyll Protein Complex)]

• B and T lymphocyte associated
• B and T lymphocyte attenuator
• B- and T-lymphocyte attenuator
• B- and T-lymphocyte-associated protein
• BTLA
• BTLA1
• CD272 antigen
• CD272
• FLJ16065
• MGC129743

Background

B and T Lymphocyte Attenuator (BTLA) also known as CD272, is a 70kDa type I transmembrane glycoprotein of the CD28 family of T cell costimulatory molecules. BTLA functions as a coinhibitor on activated T cells and on B cells and dendritic cells. The extracellular domain (ECD) of this Ig superfamily protein contains one V‑type Ig-like domain, which interacts with the herpesvirus entry mediator (HVEM/TNFSF14). The amino acid sequence of the ECD of human BTLA shares only 34% identity to that of mouse BTLA. One human BTLA variant lacking the transmembrane sequence has been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BTLA/CD272 Antibody (FAB3354C)(1)

Publication using FAB3354C

• Choi J, Maeng H, Lee S, Kim Y, Kim D, Lee H, Namgung J, Oh H, Kim T, Jeong J, Park S, Choi Y, Kang Y, Yoon S, Lee J Diagnostic value of peripheral blood immune profiling in colorectal cancer. Ann Surg Treat Res, 2018-05-29;94(6):312-321. 2018-05-29 [PMID: 29854709] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: BTLA
• Uniprot:
  • AAP44003()