BTLA/CD272 Antibody (HMBT-6B2) - Chimeric - Azide and BSA Free

• Reactivity: Mu
• Applications: WB, Flow, Func, IP, WB, B/N
• Clone: HMBT-6B2
• Clonality: Monoclonal
• Host: Armenian Hamster
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

BTLA/CD272 Antibody (HMBT-6B2) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Armenian Hamster BTLA/CD272 Antibody (HMBT-6B2) - Chimeric - Azide and BSA Free (NBP2-81117) is a recombinant monoclonal antibody validated for use in WB, Flow and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This antibody was raised by immunising Armenian hamsters with mouse BTLA/CD272:Fc fusion protein.
• Specificity: This antibody is specific for B and T lymphocyte attenuator (BTLA), which functions as a negative regulator of T cell activation and proliferation, and attenuates B cell proliferation upon associating with its known ligand, herpes virus entry mediator (HVEM).
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Armenian Hamster
• Gene: BTLA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Flow Cytometry
  • Functional
  • Immunoblotting
  • Immunoprecipitation
  • Western Blot
• Application Notes: This chimeric hamster (Armenian) antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques. This antibody has been used in immunoblot analyses to confirm that B and T lymphocyte attenuator (BTLA) regulates T cell activation through interaction with herpesvirus entry mediator (HVEM) (Sedy et al, 2005), as well as in FACS and yeast display technology to demonstrate that BTLA exhibits structural and expression polymorphisms and is highly induced in anergic CD4+ T Cells (Hurchla et al, 2005). In a mouse conjunctivitis model, treatment with this antibody during the induction phase has been shown to decrease B-cell population, upregulate Th2 cytokine production and increase the conjunctival eosinophile numbers (Ishida et al, 2012). However, treatment with this antibody during the effector phase does not affect the development of experimental conjunctivitis (Ishida et al, 2012).

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for BTLA/CD272 Antibody (HMBT-6B2) - Chimeric - Azide and BSA Free

• B and T lymphocyte associated
• B and T lymphocyte attenuator
• B- and T-lymphocyte attenuator
• B- and T-lymphocyte-associated protein
• BTLA
• BTLA1
• CD272 antigen
• CD272
• FLJ16065
• MGC129743

Background

BTLA expression is induced during activation of T cells, and BTLA remains expressed on Th1 cells but not Th2 cells. Like PD1 (MIM 600244) and CTLA4 (MIM 123890), BTLA interacts with a B7 homolog, B7H4.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BTLA