BTK Antibody (566928) [Unconjugated]

Western Blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 0.05 µg/ml of Mouse Anti-Human BTK Monoclonal Antibody (Catalog # MAB5807) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for BTK at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

BTK was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Mouse Anti-Human BTK Monoclonal Antibody (Catalog # MAB5807) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western shows lysates of Daudi human Burkitt's lymphoma cell line and K562 human chronic myelogenous leukemia cell line, loaded at 0.2 mg/ml. A specific band was detected for BTK at approximately 75 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human BTK Monoclonal Antibody (Catalog # MAB5807). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, ICC/IF
• Clone: 566928
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

BTK Antibody (566928) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human BTK
  Glu280-Val377
  Accession # Q06187
• Specificity: Detects endogenous human BTK in Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BTK
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.05 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BTK Antibody (566928) [Unconjugated]

• Agammaglobulinaemia tyrosine kinase
• AGMX1
• AGMX1MGC126262
• AT
• ATKIMD1
• B-cell progenitor kinase
• BPK
• Bruton agammaglobulinemia tyrosine kinase
• Bruton tyrosine kinase
• BTK
• dominant-negative kinase-deficient Brutons tyrosine kinase
• EC 2.7.10
• EC 2.7.10.2
• IMD1
• PSCTK1
• tyrosine-protein kinase BTK
• XLA
• XLAMGC126261

Background

Bruton tyrosine kinase (BTK) is a 75 kDa cytoplasmic protein tyrosine kinase that is widely expressed in hematopoietic cells. BTK is required for B cell receptor signaling and B cell development. Defects in BTK result in X-linked agammaglobulinemia which is characterized by a severely decreased level of circulating antibodies. Like other Tec family kinases, BTK contains a Pleckstrin homology domain, a Tec homology domain, an SH3 domain, an SH2 domain, and a protein kinase domain. Association of the BTK SH2 domain with the B cell linker protein (BLNK) is required for the activation of PLC gamma by BTK. Within the SH2 domain (aa 280‑377), human BTK shares 99% aa sequence identity with mouse and rat BTK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BTK Antibody (MAB5807)(1)

Publication using MAB5807

• Claire E. Fletcher, Eric Sulpice, Stephanie Combe, Akifumi Shibakawa, Damien A. Leach, Mark P. Hamilton et al. Androgen receptor-modulatory microRNAs provide insight into therapy resistance and therapeutic targets in advanced prostate cancer Oncogene 2019-07-01 [PMID: 31043708]

Bioinformatics

• Gene Symbol: BTK
• Uniprot:
  • Q06187()