BRF2 Antibody

Western Blot: BRF2 Antibody [NB100-1120] - (1.5ug/ml) Annlysis of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

BRF2 Antibody Summary

• Description: Novus Biologicals Goat BRF2 Antibody (NB100-1120) is a polyclonal antibody validated for use in WB and ELISA. Anti-BRF2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QAARQAATSVPNPP corresponding to C-Terminus according to NP_060780.2.
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BRF2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in Hela and human placenta lysates (predicted MW of 48 kDa band according to NP_060780).
• Control:

  HeLa Whole Cell Lysate
  BRF2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BRF2 Antibody

• B-related factor 2
• BRF-2
• BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like
• BRFUhTFIIIB50
• FLJ11052
• hBRFU
• TFIIIB50RNA polymerase III transcription initiation factor BRFU
• transcription factor IIB- related factor, TFIIIB50
• transcription factor IIIB 50 kDa subunit

Background

BRF2 is one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. BRF2 is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BRF2 Antibody (NB100-1120)(1)

Publication using NB100-1120

• Schramm L, Pendergrast PS, Sun Y, Hernandez N. Different human TFIIIB activities direct RNA polymerase III transcription from TATA-containing and TATA-less promoters. Genes Dev 2000-10-15 [PMID: 11040218]

Bioinformatics

• Gene Symbol: BRF2
• Entrez:
  • 55290(Human)
• Uniprot:
  • NP_060780.(Human)