BRF2 Antibody

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Western Blot: BRF2 Antibody [NB100-1120] - (1.5ug/ml) Annlysis of Hela lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

BRF2 Antibody Summary

Immunogen
Peptide with sequence C-QAARQAATSVPNPP corresponding to C-Terminus according to NP_060780.2.
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BRF2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 50 kDa band observed in Hela and human placenta lysates (predicted MW of 48 kDa band according to NP_060780).
Positive Control
HeLa Lysate (NB800-PC1)
BRF2 Lysate (NBL1-08027)
Publications
Read Publication using NB100-1120.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BRF2 Antibody

  • B-related factor 2
  • BRF-2
  • BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like
  • BRFUhTFIIIB50
  • FLJ11052
  • hBRFU
  • TFIIIB50RNA polymerase III transcription initiation factor BRFU
  • transcription factor IIB- related factor, TFIIIB50
  • transcription factor IIIB 50 kDa subunit

Background

BRF2 is one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. BRF2 is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BRF2 Antibody (NB100-1120)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol BRF2
Entrez
Uniprot