BRF2 Overexpression Lysate (Native)

Images

 
Western Blot: BRF2 Overexpression Lysate (Adult Normal) [NBL1-08027] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BRF2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

BRF2 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202314 and based on accession number NM_018310. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like (BRF2), mRNA.
Gene
BRF2

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
46.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for BRF2

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for BRF2 Overexpression Lysate (Native)

  • B-related factor 2
  • BRF-2
  • BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like
  • BRFUhTFIIIB50
  • FLJ11052
  • hBRFU
  • TFIIIB50RNA polymerase III transcription initiation factor BRFU
  • transcription factor IIB- related factor, TFIIIB50
  • transcription factor IIIB 50 kDa subunit

Background

This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol BRF2