Breast carcinoma amplified sequence 3 Antibody

Images

 
Western Blot: Breast carcinoma amplified sequence 3 Antibody [NB110-40682] - Detection of Human BCAS3 on HeLa whole cell lysates using NB110-40682. BCAS3 was also immunoprecipitated by another rabbit anti-BCAS3 antibody.
Immunohistochemistry-Paraffin: Breast carcinoma amplified sequence 3 Antibody [NB110-40682] - Human breast carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).
Immunohistochemistry-Paraffin: Breast carcinoma amplified sequence 3 Antibody [NB110-40682] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-BCAS3 used at a dilution of 1:500. DAB staining using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Concentration
0.2 mg/ml

Order Details

Breast carcinoma amplified sequence 3 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 870 and the C-terminus (residue 928) of human Breast Carcinoma Amplified Sequence 3 using the numbering given in entry BAB15156.1 (GeneID 54828).
Clonality
Polyclonal
Host
Rabbit
Gene
BCAS3
Purity
Immunogen affinity purified
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Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Conjugate
Unconjugated
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
This antibody is useful for Western Blot Immunohistochemistry-Paraffin and Immunoprecipitation. Epitope exposure is recommended. Epitope exposure with citrate pH6.0 buffer or tris-EDTA pH9.0 buffer will enhance staining. Likely to work with frozen sections

Reactivity Notes

Human

Alternate Names for Breast carcinoma amplified sequence 3 Antibody

  • BCAS4/BCAS3 fusion
  • breast carcinoma amplified sequence 3
  • breast carcinoma amplified sequence 4/3 fusion protein
  • breast carcinoma-amplified sequence 3
  • DKFZp686O1527
  • FLJ20128
  • GAOB1
  • MAAB
  • metastasis associated antigen of breast cancer
  • MGC4973
  • protein Maab1

Background

BCAS3 acts as an ERalpha coactivator in breast cancer cells. It has been demonstrated that PELP1, a newly described ERalpha coregulator, is recruited to BCAS3 chromatin and activates its expression. Analysis of the BCAS3 sequence for functional motifs and evidence from biochemical fractionation suggested that BCAS3 acts as a transcriptional coactivator. Results from chromatin immunoprecipitation, reporter assays, and expression studies further validated the coactivator function of BCAS3 for ERalpha. BCAS3 physically associated with histone H3 and histone acetyltransferase complex protein P/CAF and possessed histone acetyltransferase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol BCAS3
Entrez
Uniprot

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