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BRD7 Overexpression Lysate

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Western Blot: BRD7 Overexpression Lysate (Adult Normal) [NBL1-08024] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BRD7.
Western Blot: BRD7 Overexpression Lysate (Adult Normal) [NBL1-08024] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for BRD7.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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BRD7 Overexpression Lysate Summary

Description
Transient overexpression lysate of bromodomain containing 7 (BRD7) The lysate was created in HEK293T cells, using Plasmid ID RC208734 and based on accession number NM_013263. The protein contains a C-MYC/DDK Tag.
Gene
BRD7

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for BRD7 Overexpression Lysate

  • 75 kDa bromodomain protein
  • BP75
  • BP75bromodomain-containing 7
  • BRD7
  • bromodomain containing 7
  • bromodomain-containing protein 7
  • CELTIX1
  • CELTIX1NAG4
  • NAG4
  • Protein CELTIX-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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BRD7
Overexpression Lysate
NBL1-08024
BRD7 Overexpression Lysate
Species: Hu
Applications: WB

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Bioinformatics

Gene Symbol BRD7