Bradykinin RB1/BDKRB1 Antibody

Western Blot: Bradykinin RB1/BDKRB1 Antibody [NB100-96919] - Analysis of BDKRB1 using NB100-96919 at 2ug/ml in K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Bradykinin RB1/BDKRB1 Antibody Summary

• Immunogen: Peptide with sequence KVWELYKQCTPK corresponding to internal region according to NP_000701.2.
• Epitope: KVWELYKQCTPK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BDKRB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 2 - 4 ug/ml
• Application Notes: WB: Approx. 35 kDa band observed in lysates of Daudi, K562 and U937 calculated MW of 40.5 kDa band according to NP_000701.2). The observed molecular weight corresponds to earlier findings with different antibodies from other sources.
• Control:

  Bradykinin RB1/BDKRB1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Bradykinin RB1/BDKRB1 Antibody

• B1 bradykinin receptor
• B1BKR
• B1R
• BDKRB1
• BK-1 Receptor
• BKB1R
• BKR1
• BRADYB1
• bradykinin B1 receptor
• Bradykinin RB1
• bradykinin receptor 1
• bradykinin receptor B1
• BradykininRB1

Background

Bradykinin, a 9 aa peptide, is generated in pathophysiologic conditions such as inflammation, trauma, burns, shock, and allergy. Two types of G-protein coupled receptors have been found which bind bradykinin and mediate responses to these pathophysiologic conditions. The protein encoded by this gene is one of these receptors and is synthesized de novo following tissue injury. Receptor binding leads to an increase in the cytosolic calcium ion concentration, ultimately resulting in chronic and acute inflammatory responses. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bradykinin RB1/BDKRB1 Antibody (NB100-96919)(1)

Publication using NB100-96919

• Bengtson SH, Eddleston J, Christiansen SC, Zuraw BL. Double-stranded RNA increases kinin B1 receptor expression and function in human airway epithelial cells. Int Immunopharmacol 2007-12-20 [PMID: 18039525]

Bioinformatics

• Gene Symbol: BDKRB1
• Entrez:
  • 623(Human)
• Uniprot:
  • NP_000701.2(Human)