BPTF/FALZ Antibody (2F10)

Western Blot: BPTF/FALZ Antibody (2F10) [NBP2-37608] - Western blot analysis using BPTF mAb against HEK293 (1) and BPTF (AA: 503-670)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: BPTF/FALZ Antibody (2F10) [NBP2-37608] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Discontinued

BPTF/FALZ Antibody (2F10) Summary

• Immunogen: Purified recombinant fragment of human BPTF expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: BPTF
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 338 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for BPTF/FALZ Antibody (2F10)

• BPTF
• BPTF/FALZ
• bromodomain and PHD domain transcription factor
• Bromodomain and PHD finger-containing transcription factor
• bromodomain PHD finger transcription factor
• EC 3.6.1
• EC 6.2.1.5
• FAC1
• FAC1fetal Alz-50 reactive clone 1
• FALZ
• FALZnucleosome-remodeling factor subunit BPTF
• Fetal Alz-50 clone 1 protein
• Fetal Alzheimer antigennucleosome remodeling factor, large subunit
• NURF301

Background

FALZ/BPTF is a histone-binding component of NURF (nucleosome remodeling factor), a complex that catalyzes ATP-dependent nucleosome sliding and facilitates transcription of chromatin. This gene was identified in brain homogenates from patients with Alzheimer's disease. Analysis of the original protein (fetal Alz-50 reactive clone 1, or FAC1), containing a DNA-binding domain, a zinc finger motif, and a C-terminal bromodomain, suggested it might play a role in the regulation of transcription during proliferation. High levels of FAC1 were detected in fetal brain and in patients with neurodegenerative diseases. This protein is highly similar to the largest subunit of the Drosophila NURF (nucleosome remodeling factor) complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for BPTF/FALZ Antibody (NBP2-37608). (Showing 1 - 1 of 1 FAQ).

1. I am interesting by anti-BPTF antibody. I would like to know if a large protein like that could be revealed in PVDF membrane?
   • Regarding your question, nucleosome-remodeling factor subunit (BPTF) is a very large protein as you have also mentioned (338KD). There are some tips for efficient transfer of these proteins in western blot for instance: 1) Make sure to use a low percentage gel, for BPTF in particular I suggest you use 7% gel or even lower (5%). 2) You can use either Nitrocellulose or PVDF, to my knowledge the binding efficiency is higher for nitrocellulose while PVDF is a better choice of membrane if you are planning to do re-probing and stripping. If you are going to use PVDF, make sure to wet it in 100% methanol before use. Furthermore, PVDF membrane exhibits better binding efficiency of electroblotted material in the presence of SDS in the transfer buffer. However SDS added to facilitate transfer of large proteins should not exceed 0.05% as it will interfere with the binding of the protein to the membrane. 3) For transfer you can either use the semi-dry or wet transfer but you decide doing wet transfer, I strongly recommend you doing the transfer in low voltage (at 50v) for instance in order to obtain an efficient transfer. These are some suggestions I can provide for a better and more efficient transfer for large proteins like BPTF.

Bioinformatics

• Gene Symbol: BPTF