BOB1 Antibody

Western Blot: BOB1 Antibody [NB100-1122] - Analysis of BOB1 in Mouse Spleen lysate at 2 ug/ ml.

Western Blot: BOB1 Antibody [NB100-1122] - Staining (2ug/ml) of Mouse Spleen lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Mu, Hu, Rt, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

BOB1 Antibody Summary

• Description: Novus Biologicals Goat BOB1 Antibody (NB100-1122) is a polyclonal antibody validated for use in WB and ELISA. Anti-BOB1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DAYALNHTLSVEGF corresponding to C-Terminus according to NP_006226.2.
• Predicted Species: Human (100%), Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: POU2AF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 30 kDa band observed in mouse spleen lysate (predicted MW of 28 kDa band according to NP_006226).
• Control:

  BOB1 Overexpression Lysate
  Mouse Spleen Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BOB1 Antibody

• B-cell-specific coactivator OBF-1
• BOB-1
• OBF-1
• OBF1BOB1
• OCAB
• OCA-B
• OCT-binding factor 1
• POU class 2 associating factor 1
• POU domain class 2, associating factor 1
• POU domain class 2-associating factor 1
• POU domain, class 2, associating factor 1

Background

A novel 35kDa protein designated Bob1 (B cell Oct binding protein 1), alternativelt called OBF1, specifically interacts with Oct1 and Oct2, enhancing their transcriptional efficacy. Bob1 is expressed at highest levels in spleen and peripheral blood leukocytes and represents an Oct cofactor capable of conferring cell specific activation of Oct1 and Oct2. Although having no intrinsic capacity for DNA binding, Bob1 associates tightly with the octomer motif in the presence of Oct1 and Oct2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BOB1 Antibody (NB100-1122)(2)

Publications using NB100-1122

• Haines E, Nishida Y, Carr MI et al. DNA-PK inhibitor peposertib enhances p53-dependent cytotoxicity of DNA double-strand break inducing therapy in acute leukemia. Scientific reports 2021-11-01 [PMID: 34108527]
• Strubin M, Newell JW, Matthias P. OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins. Cell 1995-02-10 [PMID: 7859290]

Bioinformatics

• Gene Symbol: POU2AF1
• Entrez:
  • 5450(Human)
  • 18985(Mouse)
• Uniprot:
  • NP_006226.(Human)