BOB1 Antibody

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Western Blot: BOB1 Antibody [NB100-1122] - Analysis of BOB1 in Mouse Spleen lysate at 2 ug/ ml.
Western Blot: BOB1 Antibody [NB100-1122] - Staining (2ug/ml) of Mouse Spleen lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

BOB1 Antibody Summary

Immunogen
Peptide with sequence C-DAYALNHTLSVEGF corresponding to C-Terminus according to NP_006226.2.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
POU2AF1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 30 kDa band observed in mouse spleen lysate (predicted MW of 28 kDa band according to NP_006226).
Positive Control
BOB1 Lysate (NBL1-14616)
Spleen Lysate (NB820-59670)
Publications
Read Publication using NB100-1122.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Human, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BOB1 Antibody

  • B-cell-specific coactivator OBF-1
  • BOB-1
  • OBF-1
  • OBF1BOB1
  • OCAB
  • OCA-B
  • OCT-binding factor 1
  • POU class 2 associating factor 1
  • POU domain class 2, associating factor 1
  • POU domain class 2-associating factor 1
  • POU domain, class 2, associating factor 1

Background

A novel 35kDa protein designated Bob1 (B cell Oct binding protein 1), alternativelt called OBF1, specifically interacts with Oct1 and Oct2, enhancing their transcriptional efficacy. Bob1 is expressed at highest levels in spleen and peripheral blood leukocytes and represents an Oct cofactor capable of conferring cell specific activation of Oct1 and Oct2. Although having no intrinsic capacity for DNA binding, Bob1 associates tightly with the octomer motif in the presence of Oct1 and Oct2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BOB1 Antibody (NB100-1122)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for BOB1 Antibody (NB100-1122) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for BOB1 Antibody (NB100-1122)

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Blogs on BOB1.

Winter Protein Games
Get into the Winter Protein Games 2014! Check out the contenders competing including SKI, POLE, ICEBURG, SKT, TRAIL, WIN and BOB1. Learn more about each protein's function, gene name, molecular weight and family.View all the fun and games happening...  Read full blog post.

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Bioinformatics

Gene Symbol POU2AF1
Entrez
Uniprot