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BOB1 Antibody (BOB1/2424) [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
BOB1/2424
Clonality
Monoclonal
Host
Mouse
Conjugate
Biotin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-75753AF350
Alexa Fluor 405
NBP2-75753AF405
Alexa Fluor 488
NBP2-75753AF488
Alexa Fluor 532
NBP2-75753AF532
Alexa Fluor 594
NBP2-75753AF594
Alexa Fluor 647
NBP2-75753AF647
Alexa Fluor 700
NBP2-75753AF700
Alexa Fluor 750
NBP2-75753AF750
DyLight 350
NBP2-75753UV
DyLight 405
NBP2-75753V
DyLight 488
NBP2-75753G
DyLight 550
NBP2-75753R
DyLight 594
NBP2-75753DL594
DyLight 650
NBP2-75753C
DyLight 680
NBP2-75753FR
DyLight 755
NBP2-75753IR
FITC
NBP2-75753F
HRP
NBP2-75753H
Janelia Fluor 549
NBP2-75753JF549
Janelia Fluor 646
NBP2-75753JF646
Unconjugated
NBP2-75752-20ug
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-75752-20ug
Azide and BSA Free
NBP2-75753
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BOB1 Antibody (BOB1/2424) [Biotin] Summary

Immunogen
Recombinant human BOB1 (POU2AF1) protein fragment (around aa 148-255) (exact sequence is proprietary)
Localization
Nuclear
Specificity
BOB1 expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB1. Conversely, only some cases of classical Hodgkin lymphoma show BOB1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells. Expression of BOB1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia. B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
POU2AF1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for BOB1 Antibody (BOB1/2424) [Biotin]

  • B-cell-specific coactivator OBF-1
  • BOB-1
  • OBF-1
  • OBF1BOB1
  • OCAB
  • OCA-B
  • OCT-binding factor 1
  • POU class 2 associating factor 1
  • POU domain class 2, associating factor 1
  • POU domain class 2-associating factor 1
  • POU domain, class 2, associating factor 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: OCT2 Antibody (Oct2/2137) [NBP2-75734] - Formalin-fixed, paraffin-embedded human Tonsil stained with Oct-2 Mouse Monoclonal Antibody (OCT2/2137).
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Immunocytochemistry/Immunofluorescence: OCT1 Antibody [NBP2-21584] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: OCT1 protein stained by OCT1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by () diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 um.
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Simple Western: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Transcriptional activation by E2F4 in mouse ES cells is mediated by chromatin modifiers. Validation of interactions between GFP-E2F4 and DP-1, Host Cell Factor 1/HCFC1, YEATS2, LIN54, and LIN9, by co-immunoprecipitation followed by immunoassay, in mouse ES cells and human RPE cells. Transcriptional activators (Host Cell Factor 1/HCFC1 and YEATS2) preferentially bind to E2F4 in mouse ES cells while members of the DREAM repressor complex (LIN54 and LIN9) bind preferentially to E2F4 in RPE cells. Molecular weights (kDa) are indicated on the left side (one experiment shown of at least two experiments) Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41467-019-10901-x), licensed under a CC-BY licence.
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Immunohistochemistry-Paraffin: SLC22A2/OCT2 Antibody [NBP1-89417] - Analysis in human kidney and liver tissues. Corresponding SLC22A2 RNA-seq data are presented for the same tissues.
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NBP2-38790
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Immunohistochemistry-Paraffin: Pax5/BSAP Antibody [NBP2-38790] - Paraffin-embedded alcohol fixed rat spleen tissue (40x). Antigen retrieval pH9. PAX5 dilution 1:500 overnight at 4C. IHC-P image submitted by a verified customer review.
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Immunohistochemistry-Paraffin: Bcl-6 Antibody [NBP2-59786] - Staining of human lymphoma, non-Hodgkin's type, shows strong nuclear positivity in tumor cells.
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BOB1 Antibody
(BOB1/2424) [Biotin]
NBP2-75753B
BOB1 Antibody (BOB1/2424) [Biotin]
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P

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Product General Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-75753AF350
Alexa Fluor 405 NBP2-75753AF405
Alexa Fluor 488 NBP2-75753AF488
Alexa Fluor 532 NBP2-75753AF532
Alexa Fluor 594 NBP2-75753AF594
Alexa Fluor 647 NBP2-75753AF647
Alexa Fluor 700 NBP2-75753AF700
Alexa Fluor 750 NBP2-75753AF750
Biotin NBP2-75753B
DyLight 350 NBP2-75753UV
DyLight 405 NBP2-75753V
DyLight 488 NBP2-75753G
DyLight 550 NBP2-75753R
DyLight 594 NBP2-75753DL594
DyLight 650 NBP2-75753C
DyLight 680 NBP2-75753FR
DyLight 755 NBP2-75753IR
FITC NBP2-75753F
HRP NBP2-75753H
Janelia Fluor 549 NBP2-75753JF549
Janelia Fluor 646 NBP2-75753JF646
Learn about Custom Antibody Labeling

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Bioinformatics

Gene Symbol POU2AF1