BNP Antibody (5-E2/50E1)

Product Discontinued

BNP Antibody (5-E2/50E1) Summary

• Immunogen: Human synthetic Brain natriuretic hormone
• Localization: Secreted
• Specificity: NB100-62133 detects an epitope located at the C-terminal (amino acids 26-32) of the human brain natriuretic peptide (BNP). This clone also known as 50E1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NPPB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunoassay
  • In vitro assay
  • Western Blot 1:100-1:2000
• Application Notes: Antibody detects a band of approximately 4kDa when using synthetic BNP and a band of 15kDa when using recombinant proBNP. Use in In vitro reported in scientific literature (PMID: 27329291).
• Control:

  BNP Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for BNP Antibody (5-E2/50E1)

• BNF
• BNP
• brain type natriuretic peptide
• Gamma-brain natriuretic peptide
• natriuretic peptide B
• natriuretic peptide precursor B
• natriuretic peptides B
• natriuretic protein
• NPPB

Background

Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C-type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17 amino acid ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (eleven of the 17 amino acid residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32 amino acid peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BNP Antibody (NB100-62133)(2)

Publications using NB100-62133

• Solomon T, Smith EN, Matsui H et al. Associations Between Common and Rare Exonic Genetic Variants and Serum Levels of Twenty Cardiovascular-Related Proteins: The Tromso Study. Circ Cardiovasc Genet 2016-06-21 [PMID: 27329291] (In vitro, Human)
• Bu D, Zhou Y, Tang J et al. Expression and purification of a novel therapeutic single-chain variable fragment antibody against BNP from inclusion bodies of Escherichia coli. Protein Expr Purif. 2013-12-01 [PMID: 24128692] (WB, Human)

Bioinformatics

• Gene Symbol: NPPB
• Entrez:
  • 4879(Human)
• Uniprot:
  • P16860()