BNIP1 Antibody

Western Blot: BNIP1 Antibody [NB100-68161] - (0.5ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

BNIP1 Antibody Summary

• Description: Novus Biologicals Goat BNIP1 Antibody (NB100-68161) is a polyclonal antibody validated for use in WB and ELISA. Anti-BNIP1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DLLRQRKTTKESLAQ corresponding to internal region according to NP_053581.1, NP_053582.1, NP_053583.1.
• Epitope: DLLRQRKTTKESLAQ
• Specificity: All reported variants represent isoform 1 (NP_001196.1; NP_053581.1; NP_053582.1; NP_053583.1)
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BNIP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:8000
  • Western Blot 0.5 - 1.5 ug/ml
• Application Notes: WB: Approx. 28 kDa band observed in human kidney lysates (calculated MW of 26.1 kDa band according to NP_001196.1; NP_053581.1; NP_053582.1; NP_053583.1). An additional band of unknown identity was also consistently observed at 48 kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Control:

  BNIP1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BNIP1 Antibody

• BCL2/adenovirus E1B 19 kDa protein-interacting protein 1
• BCL2/adenovirus E1B 19kDa interacting protein 1
• BCL2/adenovirus E1B 19kD-interacting protein 1
• Nip1
• SEC20
• SEC20L
• Transformation-related gene 8 protein
• TRG-8
• vesicle transport protein SEC20

Background

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. Alternative splicing of this gene results in four protein products with identical N- and C-termini.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BNIP1 Antibody (NB100-68161)(1)

Publication using NB100-68161

• Nakajima K, Hirose H, Taniguchi M et al. Involvement of BNIP1 in apoptosis and endoplasmic reticulum membrane fusion. EMBO J 2004-08-18 [PMID: 15272311]

Bioinformatics

• Gene Symbol: BNIP1
• Entrez:
  • 662(Human)
  • 224630(Mouse)
  • 140932(Rat)
• Uniprot:
  • NP_001196.1(Human)