Human BMP-5 Recombinant Protein

Product Discontinued

Human BMP-5 Recombinant Protein Summary

• Description: A disulfide-linked homodimeric protein consisting two 138 residues glycosylated chains and corresponding to amino acids Ala317 - His454 of BMP5 (P22003).
  
  

  Source: CHO cell line

• Preparation Method: Lyophilized from 0.2 mm filtered concentrated solution in 4 mM HCl.
• Details of Functionality: The ED50, as determined by inducing alkaline phosphatase production using mouse ATDC5 cells, is less than 1.2 mg/mL.
• Source: CHO
• Protein/Peptide Type: Recombinant Protein
• Gene: BMP5
• Endotoxin Note: Less than 1 EU/mg of the rHuBMP-5 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
  • SDS-Page

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: LYOPH
• Reconstitution Instructions: Centrifuge prior to opening. Reconstitute with 4 mM HCl to a final concentration of 0.1 mg/ml. Further dilutions should be made in appropriately buffered solutions.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Human BMP-5 Recombinant Protein

• BMP5
• BMP-5
• bone morphogenetic protein 5
• MGC34244

Background

Bone Morphogenetic Protein-5 (BMP-5) is a TGF-b superfamily cytokine that forms 40 kDa disulfide-linked homodimers. It signals through hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors. BMP-5 promotes chondrocyte proliferation and differentiation, ovarian granulosa cell proliferation, dopaminergic neuronal differentiation, and dendrite outgrowth.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: BMP5