BMI-1 Antibody (BLR119H)

Immunoprecipitation: BMI-1 Antibody [NBP3-14704] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from HEK293T cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiBMI1 recombinant monoclonal antibody used for IP at 6 ul/mg lysate. BMI1 wasalso immunoprecipitated by rabbit anti-BMI1 antibodies and For blottingimmunoprecipitated BMI1, was used at 1:1000.Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: BLR119H
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

BMI-1 Antibody (BLR119H) Summary

• Additional Information: Recombinant Monoclonal
• Immunogen: between 250 and 300
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BMI1
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with , BSA Free
• Preservative: 0.09% Sodium Azide
• Purity: >95%

Alternate Names for BMI-1 Antibody (BLR119H)

• B lymphoma Mo-MLV insertion region 1 homolog (mouse)
• B lymphoma Mo-MLV insertion region 1 homolog
• BMI1 polycomb ring finger oncogene
• BMI1
• BMI-1
• FLVI2/BMI1
• MGC12685
• murine leukemia viral (bmi-1) oncogene homolog
• PCGF4
• polycomb complex protein BMI-1
• polycomb group protein Bmi1
• polycomb group ring finger 4
• RING finger protein 51
• RNF51
• RNF51Polycomb group RING finger protein 4

Background

Bmi1 polycomb ring finger oncogene regulates the cell cycle inhibitor genes p16 and p19, with implications in hematopoiesis, skeletal patterning, neurological functions, and development of the cerebellum. Bmi 1 has been shown to induce telomerase activity and immortalize human mammary epithelial cells, and extend the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. Bmi-1 is also required for efficient self-renewing cell division in adult hematopoietic and neural stem cells.

Aberrant expression of Bmi-1 has been found in several types of cancer in the bladder, skin, blood, prostate, breast, ovaries, andcolon. Its overexpression is especially pronounced in mantle cell lymphomas. Thereforem Bmi1 antibodies will be useful tools for many cancer development studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BMI1