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BMI-1 Antibody (3E3)

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Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against human Bmi1 (AA: 1-326) recombinant protein. (Expected MW is 74 kDa)
Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against human Bmi1 (AA: 1-326) recombinant protein. (Expected MW is 74 kDa)
Immunocytochemistry/ Immunofluorescence: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of Hela cells using Bmi1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa ...read more
Immunohistochemistry-Paraffin: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of paraffin-embedded rectum cancer tissues using Bmi1 mouse mAb with DAB staining.
Flow Cytometry: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of NIH/3T3 cells using Bmi1 mouse mAb (green) and negative control (red).
Western Blot: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis using Bmi1 mAb against HEK293 (1) and BMI1-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: BMI-1 Antibody (3E3) [NBP2-22204] - Analysis of paraffin-embedded cervical cancer tissues using Bmi1 mouse mAb with DAB staining.
ELISA: BMI-1 Antibody (3E3) [NBP2-22204] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
3E3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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BMI-1 Antibody (3E3) Summary

Immunogen
Purified recombinant fragment of human Bmi1 expressed in E. Coli.
Localization
Nucleus, Cytoplasm
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
BMI1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1:1000
Theoretical MW
37 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for BMI-1 Antibody (3E3)

  • B lymphoma Mo-MLV insertion region 1 homolog (mouse)
  • B lymphoma Mo-MLV insertion region 1 homolog
  • BMI1 polycomb ring finger oncogene
  • BMI1
  • BMI-1
  • FLVI2/BMI1
  • MGC12685
  • murine leukemia viral (bmi-1) oncogene homolog
  • PCGF4
  • polycomb complex protein BMI-1
  • polycomb group protein Bmi1
  • polycomb group ring finger 4
  • RING finger protein 51
  • RNF51
  • RNF51Polycomb group RING finger protein 4

Background

Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: p19ARF/CDKN2A Antibody [NB200-106] - Analysis of p16INK4a/CDKN2A in (A) NIH/3T3 and (B) MEF cells.
Immunocytochemistry/Immunofluorescence: p19ARF/CDKN2A Antibody [NB200-106] - Antibody was tested in MEF cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).
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Western Blot: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - BMI-1 expression in human cell lines (MCF-7, K562 and A459). Image from verified customer review.
Immunocytochemistry/Immunofluorescence: Bmi1 Antibody (LLBmi1-1) [NBP1-96140] - Bmi1 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).
Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on U2OS cells with BMI-1 Antibody [LLBmi1-1] NBP1-96140AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
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Immunohistochemistry-Paraffin: c-Myc Antibody (9E10) [NB600-302] - c-Myc was detected in immersion fixed paraffin-embedded sections of human breast cancer using anti-human mouse monoclonal antibody (Catalog # NB600-302, clone 9E10) at 1:50 dilution overnight at 4 C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.
Simple Western: c-Myc Antibody (9E10) [NB600-302] - Simple Western lane view shows a specific band for c-Myc in 0.5 mg/ml of Jurkat lysate. This experiment as performed under reducing conditions using the 12-230 kDa separation system.
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Western Blot: PRC1 Antibody [NBP1-88856] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunohistochemistry-Paraffin: PRC1 Antibody [NBP1-88856] - Staining of human cerebral cortex shows moderate positivity in nuclear membrane in neurons.
Immunohistochemistry-Paraffin: PRC1 Antibody [NBP1-88856] - Staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.
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BMI-1 Antibody
(3E3)
NBP2-22204
BMI-1 Antibody (3E3)
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P

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