BMI-1 Antibody (384509)

Images

 
BMI‑1 was detected in immersion fixed paraffin-embedded sections of normal human breast using Human BMI‑1 Monoclonal Antibody (Catalog # MAB33342) at 15 µg/mL overnight at 4 °C. Tissue was stained using the ...read more
BMI‑1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Human BMI‑1 Monoclonal Antibody (Catalog # MAB33342) at 25 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
384509
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

BMI-1 Antibody (384509) Summary

Immunogen
E. coli-derived recombinant human BMI-1
Asp96-Gly326
Accession # P35226
Specificity
Detects human BMI-1 in direct ELISAs.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
BMI1
Purity
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB33342 in the following application:

Publications
Read Publications using
MAB33342 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BMI-1 Antibody (384509)

  • B lymphoma Mo-MLV insertion region 1 homolog (mouse)
  • B lymphoma Mo-MLV insertion region 1 homolog
  • BMI1 polycomb ring finger oncogene
  • BMI1
  • BMI-1
  • FLVI2/BMI1
  • MGC12685
  • murine leukemia viral (bmi-1) oncogene homolog
  • PCGF4
  • polycomb complex protein BMI-1
  • polycomb group protein Bmi1
  • polycomb group ring finger 4
  • RING finger protein 51
  • RNF51
  • RNF51Polycomb group RING finger protein 4

Background

BMI-1 (B cell-specific Moloney-MLV integration site #1) is a 45 kDa protooncogene that is a class II member of the Polycomb group of genes. It participates in the formation of a large multimeric complex termed PRC1 that inhibits target gene transcription. Loss of BMI-1 function precludes stem cells from self-replicating. Human BMI-1 contains an N-terminal RING-finger domain (aa 17-56), an NLS (aa 81-95) and a C-terminal Pro/Ser-rich region (aa 251-326). Human BMI-1 is 99%, 97%, 99% and 99% aa identical to bovine, mouse, feline and canine BMI-1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MAB33342
Species: Hu
Applications: IHC

Publications for BMI-1 Antibody (MAB33342)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC, Western Blot.


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IHC
(1)
Western Blot
(1)
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Filter By Species
Human
(2)
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Review for BMI-1 Antibody (MAB33342) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB33342:
Filter by Applications
IHC-P
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Human 02/20/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 22730165
SpeciesHuman
Special ApplicationsIndependent review from antibodyresource.comObtained significative results in the evaluation of laryngeal staminal cancer cells and applied the antibody also in other neoplastic samples (published in PMID 22730165).

FAQs for BMI-1 Antibody (MAB33342) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

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Bioinformatics Tool for BMI-1 Antibody (MAB33342)

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Diseases for BMI-1 Antibody (MAB33342)

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Blogs on BMI-1

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Recent Reviews

5
5
1
4
0
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0
2
0
1
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Anonymous
02/20/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol BMI1
Uniprot