BMI-1 Antibody (384509) [Alexa Fluor® 647]

• Applications: IHC
• Clone: 384509
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

BMI-1 Antibody (384509) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human BMI-1
  Asp96-Gly326
  Accession # P35226
• Specificity: Detects human BMI-1 in direct ELISAs.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

BMI-1 (B cell-specific Moloney-MLV integration site #1) is a 45 kDa protooncogene that is a class II member of the Polycomb group of genes. It participates in the formation of a large multimeric complex termed PRC1 that inhibits target gene transcription. Loss of BMI-1 function precludes stem cells from self-replicating. Human BMI-1 contains an N-terminal RING-finger domain (aa 17-56), an NLS (aa 81-95) and a C-terminal Pro/Ser-rich region (aa 251-326). Human BMI-1 is 99%, 97%, 99% and 99% aa identical to bovine, mouse, feline and canine BMI-1, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.