BMAL1 Antibody - BSA Free

Western Blot: BMAL1 Antibody [NBP2-15599] - Analysis of ARNTL expression in transfected 293T cell line by ARNTL polyclonal antibody. A: Non-transfected lysate. B: ARNTL transfected lysate. 7. 5% SDS PAGE gel, diluted at 1:500.

Immunocytochemistry/Immunofluorescence: BMAL1 Antibody [NBP2-15599] - A375 cells. Green: MOP3 protein stained by MOP3 antibody [N1N3] Blue: Hoechst 33342 staining.

Western Blot: BMAL1 Antibody [NBP2-15599] - Sample (50 ug of whole cell lysate) A: Mouse Brain, 7. 5% SDS PAGE gel, diluted at 1:1000.

Western Blot: BMAL1 Antibody [NBP2-15599] - Treated 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with BMAL1 antibody [N1N3] and PER2 antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, RM, Sh, Xp
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

BMAL1 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human BMAL1. The exact sequence is proprietary.
• Localization: Nucleus
• Predicted Species: Rat (99%), Porcine (99%), Bovine (100%), Rhesus Macaque (100%), Chicken (97%), Sheep (100%), Xenopus (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BMAL1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (96%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for BMAL1 Antibody - BSA Free

• ARNTL
• aryl hydrocarbon receptor nuclear translocator-like protein 1
• aryl hydrocarbon receptor nuclear translocator-like
• basic-helix-loop-helix-PAS orphan MOP3
• BHLHE5
• bHLHe5brain and muscle
• bHLH-PAS protein JAP3
• BMAL1
• BMAL1c
• BMAL1TIC
• Brain and muscle ARNT-like 1
• Class E basic helix-loop-helix protein 5
• JAP3
• Member of PAS protein 3
• member of PAS superfamily 3
• MOP3
• MOP3BMAL1c
• PAS domain-containing protein 3
• PASD3
• PASD3MGC47515
• TIC

Background

The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This complex binds an E-box upstream of the PER1 gene, activating this gene and possibly other circadian rhythym-associated genes. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: BMAL1
• Uniprot:
  • O00327()