Western Blot: Blooms Syndrome Protein Blm [p Thr99] Antibody [NBP1-46851] - Western blot analysis of Blooms Syndrome Protein Blm phosphorylated at Thr99 in MDA-MB-231 cells A) not treated and B) treated with 1uM ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
1M Tris/HCl (pH 8.5) and 0.2M Glycine
0.1% Sodium Azide
Immunogen affinity purified
Western Blot 1:1000
This Blooms Syndrome Protein Blm [phospho Thr99] is useful in Western blot where a band can be seen at ~160 kDa. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
160 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP1-46851 in the following applications:
Alternate Names for Blooms Syndrome Protein Blm [p Thr99] Antibody
Bloom syndrome, RecQ helicase-like
BSBloom syndrome protein
DNA helicase, RecQ-like type 2
RecQ protein-like 3
Blooms Syndrome Protein Blm, also called DNA helicase RecQ-like type 2 (RecQ2), plays key role in DNA replication and repair. RecQ2 exhibits Mg2+/ATP-dependent DNA-helicase activity which unwind single- and double-stranded DNA in a 3'-5' direction. RecQ2 implicates in 5'-end resection of DNA during double-strand breaks repair: unwinds DNA and recruits DNA2 which mediates the cleavage of 5'-ssDNA. RecQ2 forms a part of the BRCA1-associated genome surveillance complex (BASC, contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2) and RAD50-MRE11-NBS1 protein complex. RecQ2 also interacts with ubiquitinated FANCD2, SUPV3L1 and RMI1 of RMI complex. RecQ2 is localized in the nucleus and gets phosphorylated in response to DNA damage. Phosphorylation requires the FANCA-FANCC-FANCE-FANCF-FANCG protein complex, as well as the presence of RMI1. RecQ2 has been linked to the etiology of Bloom syndrome (BLM), an autosomal recessive disorder characterized by proportionate pre- and postnatal growth deficiency, sun-sensitive telangiectatic hypo- and hyperpigmented skin, predisposition to malignancy, and chromosomal instability
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.