Blood group H inhibitor Overexpression Lysate

Images

 
Western Blot: Blood group H inhibitor Overexpression Lysate (Adult Normal) [NBL1-10863] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Blood group H inhibitor.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Blood group H inhibitor Overexpression Lysate Summary

Description

Blood group H inhibitor Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC210361

Accession#: NM_000148

Protein Tag: C-MYC/DDK
Gene
FUT1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
41.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Blood group H inhibitor Overexpression Lysate

  • alpha (1,2) fucosyltransferase
  • Alpha(1,2)FT 1,2-alpha-L-fucosyltransferase
  • Blood group H alpha 2-fucosyltransferase
  • EC 2.4.1.69
  • fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase)
  • fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase, Bombayphenotype included)
  • fucosyltransferase 1 (galactoside 2-alpha-L-fucosyltransferase, H blood group)
  • Fucosyltransferase 1
  • galactoside 2-alpha-L-fucosyltransferase 1
  • GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 1
  • HHH
  • HSC

Background

The protein encoded by this gene is a Golgi stack membrane protein that is involved in the creation of a precursor of the H antigen, which is required for the final step in the soluble A and B antigen synthesis pathway. This gene is one of two encoding the galactoside 2-L-fucosyltransferase enzyme. Mutations in this gene are a cause of the H-Bombay blood group. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

1845-SH/CF
Species: Hu
Applications: BA
NB600-1071
Species: Hu(-), Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NB600-600
Species: Ca, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, KD, WB
NBP1-47945
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-06699
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, PEP-ELISA, WB
NBP3-04509
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP3-06699
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, PEP-ELISA, WB
255-SC/CF
Species: Hu
Applications: BA
7268-CT
Species: Hu
Applications: BA
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
D6050
Species: Hu
Applications: ELISA
AF1356
Species: Hu, Mu
Applications: CyTOF-ready, Flow, IHC, Simple Western, WB
210-TA
Species: Hu
Applications: BA
AF1705
Species: Mu
Applications: IHC, WB
NBP1-92172
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-33581
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P

Publications for Blood group H inhibitor Lysate (NBL1-10863) (0)

There are no publications for Blood group H inhibitor Lysate (NBL1-10863).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Blood group H inhibitor Lysate (NBL1-10863) (0)

There are no reviews for Blood group H inhibitor Lysate (NBL1-10863). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Blood group H inhibitor Lysate (NBL1-10863) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Blood group H inhibitor Products

Bioinformatics Tool for Blood group H inhibitor Lysate (NBL1-10863)

Discover related pathways, diseases and genes to Blood group H inhibitor Lysate (NBL1-10863). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Blood group H inhibitor Lysate (NBL1-10863)

Discover more about diseases related to Blood group H inhibitor Lysate (NBL1-10863).
 

Pathways for Blood group H inhibitor Lysate (NBL1-10863)

View related products by pathway.

PTMs for Blood group H inhibitor Lysate (NBL1-10863)

Learn more about PTMs related to Blood group H inhibitor Lysate (NBL1-10863).

Blogs on Blood group H inhibitor

There are no specific blogs for Blood group H inhibitor, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Blood group H inhibitor Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol FUT1