BIN3 Antibody (3A4) - BSA Free

Western Blot: BIN3 Antibody (3A4) [NBP2-21676] - Mouse Anti-BIN3 antibody. Lane 1: MEF cells wild type. Lane 2: MEF cells null mice. Load: 35 ug per lane. BIN3 antibody overnight at 4C. Secondary antibody: Dye800 mouse secondary antibody for 45 min at RT. Block: 5% Blocking Buffer overnight at 4C. Predicted/Observed size: 29.7 kDa, 30 kDa for BIN-3. Other band: non-specific.

Western Blot of Mouse anti-BIN3 antibody. Lane 1: MEF lysate BIN3 +/-. Lane 2: MEF lysate BIN3 -/-. Load: 35 ug per lane. Primary antibody: BIN-3 antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800(TM) mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 29.7 kDa, 31 kDa for BIN 3. Other band(s): * non-specifics.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IP
• Clone: 3A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

BIN3 Antibody (3A4) - BSA Free Summary

• Description: This antibody was purified from clarified mouse ascetic fluid by Protein A chromatography followed by extensive dialysis against the buffer stated above
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: BIN3 Antibody (3A4) was produced in mouse by repeated immunizations with a full length human BIN3 recombinant protein followed by hybridoma development. (Uniprot: Q9NQY0)
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BIN3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:50000
  • Immunoprecipitation 10-100 ul
  • Western Blot 1:500-1:1500
• Application Notes: This product has been tested for use in ELISA, Western Blot, and IP. Specific conditions for reactivity should be optimized by the end user.

Reactivity Notes

Cross-reactivity with BIN3 from other sources has not been determined

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Alternate Names for BIN3 Antibody (3A4) - BSA Free

• bridging integrator 3
• MGC14978

Background

BIN3, also known as Bridging integrator 3, is a 253 amino acid protein that plays a role in cell membrane shape and structure through interaction with GTPases, and members of the BAR domain protein family have been shown to be involved in processes such as intracellular transport and endocytosis. This protein is currently being studied for research on the following diseases and disorders, ornithosis, pancreatic cancer, and pancreatitis. Interactions with the BIN3 protein have been shown to involve DNMBP, GRPEL1, VDAC3, POR, and RAD23B, CAP2B, and ERH in cell cycle, actin filament organization, cytokinesis, protein localization, and barrier septum assembly pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BIN3
• Uniprot:
  • Q9NQY0(Human)