Western Blot: BHMT Antibody [NBP1-00161] - Analysis of BHMT in Rat Liver lysate (35ug protein in RIPA buffer) using this antibody at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: BHMT Antibody [NBP1-00161] - HEK293 overexpressing BHMT and probed with this antibody (mock transfection in first lane).
WB: Approx. 45 kDa band observed in human liver, mouse liver and rat liver lysates (calculated MW of 44.9 kDa band according to human NP_001704.1, 45.0 kDa band according to mouse NP_057877.1 and rat NP_110477.1). In transfected HEK293 transiently expressing BHMT a band of approx. 49 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human kidney shows strong cytoplasm staining in PCT cells.
Read Publications using NBP1-00161 in the following applications:
Predicted cross-reactivity based on sequence identity: Canine.
Alternate Names for BHMT Antibody
betaine homocysteine methyltransferase
betaine--homocysteine S-methyltransferase 1
This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.