BHMT Antibody

Images

 
Western Blot: BHMT Antibody [NBP1-00161] - (0.03ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: BHMT Antibody [NBP1-00161] - HEK293 overexpressing BHMT and probed with this antibody (mock transfection in first lane).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

BHMT Antibody Summary

Immunogen
Peptide with sequence C-EQQLKELFEKQK corresponding to C-Terminus according to NP_001704.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BHMT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.03 ug/ml
  • Immunohistochemistry 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 45 kDa band observed in human liver, mouse liver and rat liver lysates (calculated MW of 44.9 kDa band according to human NP_001704.1, 45.0 kDa band according to mouse NP_057877.1 and rat NP_110477.1). In transfected HEK293 transiently expressing BHMT a band of approx. 49 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human kidney shows strong cytoplasm staining in PCT cells.
Positive Control
BHMT Lysate (NBL1-07976)
Publications
Read Publications using
NBP1-00161 in the following applications:

  • WB
    2 publications

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BHMT Antibody

  • betaine homocysteine methyltransferase
  • betaine-homocysteine methyltransferase
  • betaine--homocysteine S-methyltransferase 1
  • betaine--homocysteine S-methyltransferase
  • BHMT1
  • EC 2.1.1
  • EC 2.1.1.5

Background

This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

H00000875-M01
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, IHC-P, IP, RNAi
NB100-791
Species: Hu
Applications: WB, IHC, PEP-ELISA
NBP1-57749
Species: Hu
Applications: WB, IHC
NBP2-37607
Species: Hu, Rt
Applications: WB, ELISA, IHC
AF7895
Species: Hu
Applications: WB, IHC
NBP1-85826
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-59580
Species: Hu, Rt
Applications: WB
NBP1-82612
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-59904
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-87508
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-81838
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NB120-22711
Species: Hu, Mu
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
AF6526
Species: Hu
Applications: WB
AF6466
Species: Hu
Applications: WB
NBP2-32173
Species: Hu, Mu
Applications: WB, ELISA, IHC-P, IP
NBP2-34390
Species: Hu
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
NBP2-23610
Species: Hu
Applications: ICC/IF (-), WB, Flow, IHC

Publications for BHMT Antibody (NBP1-00161)(4)

Reviews for BHMT Antibody (NBP1-00161) (0)

There are no reviews for BHMT Antibody (NBP1-00161). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for BHMT Antibody (NBP1-00161) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional BHMT Products

Bioinformatics Tool for BHMT Antibody (NBP1-00161)

Discover related pathways, diseases and genes to BHMT Antibody (NBP1-00161). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for BHMT Antibody (NBP1-00161)

Discover more about diseases related to BHMT Antibody (NBP1-00161).
 

Pathways for BHMT Antibody (NBP1-00161)

View related products by pathway.

PTMs for BHMT Antibody (NBP1-00161)

Learn more about PTMs related to BHMT Antibody (NBP1-00161).

Blogs on BHMT

There are no specific blogs for BHMT, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our BHMT Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol BHMT
Entrez
Uniprot