BHMT Antibody

Images

 
Western Blot: BHMT Antibody [NBP1-00161] - Rat Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-00161] - Human Kidney tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: BHMT Antibody [NBP1-00161] - HEK293 overexpressing BHMT and probed with this antibody (mock transfection in first lane).
Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-00161] - Human Liver tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

BHMT Antibody Summary

Immunogen
Peptide with sequence C-EQQLKELFEKQK corresponding to C-Terminus according to NP_001704.1 .
Predicted Species
Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BHMT
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.03 - 0.1 ug/mL
Application Notes
Approx 45 kDa band observed in Rat Liver lysates (calculated MW of 44.9 kDa according to human NP_001704.1, 45.0 kDa according to mouse NP_057877.1 and rat NP_110477.1). This product has been successfully used in WB on Mouse (PMID:28605831, 23807810 and 22209966). In transfected HEK293 transiently expressing BHMT a band of approx. 49kDa is observed. This band is not observed in the non-transfected HEK293.
Control
BHMT Overexpression Lysate
Publications
Read Publications using
NBP1-00161 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for BHMT Antibody

  • betaine homocysteine methyltransferase
  • betaine-homocysteine methyltransferase
  • betaine--homocysteine S-methyltransferase 1
  • betaine--homocysteine S-methyltransferase
  • BHMT1
  • EC 2.1.1
  • EC 2.1.1.5

Background

This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for BHMT Antibody (NBP1-00161)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for BHMT Antibody (NBP1-00161) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for BHMT Antibody (NBP1-00161)

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Bioinformatics

Gene Symbol BHMT
Entrez
Uniprot