beta Tubulin Antibody (TU-06)

Images

 
Western Blot: beta Tubulin Antibody (TU-06) [NB120-7792] - Analysis (reducing conditions) of HPB-ALL human peripheral blood leukemia cell line. Lane 1: negative control. Lane 2,3,4,5,6: immunostaining with ...read more
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (TU-06) [NB120-7792] - Staining of 3T3 mouse embryonal fibroblast cell line using anti-beta-tubulin (TU-06) (detection by Goat anti-mouse IgM Cy(R)5). ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Ch, Fi, PlSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, Single-Cell Western
Clone
TU-06
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

beta Tubulin Antibody (TU-06) Summary

Immunogen
Beta-subunits of porcine brain tubulin.
Localization
Cytoplasmic
Marker
Microtubule Marker
Specificity
The antibody TU-06 recognizes an epitope (aa 81-95) on phylogenetically conserved N-terminal structural domain of beta-tubulin (recognizes all beta-tubulin isoforms) in various species.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
TUBB
Purity
Multi-step
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Single Cell Western
  • Western Blot 1 ug/ml
Application Notes
Western Blot: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions. Immunocytochemistry - Staining technique: fixed and permeabilized cells
Reviewed Applications
Read 1 Review rated 4
using
NB120-7792 in the following applications:

Publications
Read Publications using
NB120-7792 in the following applications:

  • WB
    2 publications

Reactivity Notes

Expected species cross-reactivity based on sequence homology: Carp, Bovine, Hamster, S.Cerevisiae, S.Pombe, and Protozoa. Plant reactivity reported in scientific literature (PMID: 26594217).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS (pH 8.0)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Multi-step

Alternate Names for beta Tubulin Antibody (TU-06)

  • beta polypeptide
  • MGC117247
  • Tubb5
  • tubulin beta chain
  • tubulin beta polypeptide
  • tubulin beta-1 chain
  • Tubulin beta-5 chain
  • tubulin, beta

Background

Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, ICC/IF, IHC, IHC-P, Single-Cell Western

Publications for beta Tubulin Antibody (NB120-7792)(2)

Review for beta Tubulin Antibody (NB120-7792) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NB120-7792:
Filter by Applications
WB
(1)
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Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
David Riddick
WB Rat 01/22/2014
View

Summary

ApplicationWestern Blot
Sample Testedrat liver cytosol
SpeciesRat

Blocking

Blocking Detailsskim milk powder in TNT

Primary Anitbody

Dilution Ratio1/10,000

Secondary Antibody

Secondary Descriptiongoat anti-mouse IgG / HRP

Details

Detection Notesenhanced chemiluminescence

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for beta Tubulin Antibody (NB120-7792) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for beta Tubulin Antibody (NB120-7792)

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Blogs on beta Tubulin

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
David Riddick
01/22/2014
Application: WB
Species: Rat

Bioinformatics

Gene Symbol TUBB
Entrez
Uniprot