Western Blot: beta Tubulin Antibody (TU-06) [NB120-7792] - Analysis (reducing conditions) of HPB-ALL human peripheral blood leukemia cell line. Lane 1: negative control. Lane 2,3,4,5,6: immunostaining with ...read more
Immunocytochemistry/ Immunofluorescence: beta Tubulin Antibody (TU-06) [NB120-7792] - Staining of 3T3 mouse embryonal fibroblast cell line using anti-beta-tubulin (TU-06) (detection by Goat anti-mouse IgM Cy(R)5). ...read more
Western Blot: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer. Application note: Reducing conditions. Immunocytochemistry - Staining technique: fixed and permeabilized cells
Read 1 Review rated 4 using NB120-7792 in the following applications:
Expected species cross-reactivity based on sequence homology: Carp, Bovine, Hamster, S.Cerevisiae, S.Pombe, and Protozoa. Plant reactivity reported in scientific literature (PMID: 26594217).
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
TBS (pH 8.0)
15mM Sodium Azide
Alternate Names for beta Tubulin Antibody (TU-06)
tubulin beta chain
tubulin beta polypeptide
tubulin beta-1 chain
Tubulin beta-5 chain
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.