beta Sarcoglycan Antibody

Western Blot: beta Sarcoglycan Antibody [NBP3-12235] - WB. 1:500 antibody dilution in Diluobuffer. Apparent MW is 76 kDa

Immunohistochemistry-Paraffin: beta Sarcoglycan Antibody [NBP3-12235] - Soluble guanylate cyclase beta 1. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification on Leica DM4000 microscope. FFPE sections.

Immunohistochemistry-Paraffin: beta Sarcoglycan Antibody [NBP3-12235] - Rat cerebellum-Soluble guanylate beta 1. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 5X magnification on leica DM4000 microscope. FFPE section.

Immunohistochemistry-Paraffin: beta Sarcoglycan Antibody [NBP3-12235] - Soluble guanylate cyclase beta 1. 1:100 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnification on Leica DM4000 microscope. FFPE sections.

Product Discontinued

beta Sarcoglycan Antibody Summary

• Immunogen: Synthetic peptide taken within amino acid region 175-225 on rat guanylate cyclase soluble subunit beta-1 protein.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SGCB
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 1:200
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for beta Sarcoglycan Antibody

• 43 kDa dystrophin-associated glycoprotein
• 43DAG
• A3b
• A3bbeta-SG
• beta Sarcoglycan
• beta-Sarcoglycan
• beta-sarcoglycan(43kD dystrophin-associated glycoprotein)
• Beta-SG
• LGMD2E
• limb girdle muscular dystrophy 2E (non-linked families)
• Sarcoglycan beta
• sarcoglycan, beta (43kD dystrophin-associated glycoprotein)
• sarcoglycan, beta (43kDa dystrophin-associated glycoprotein)
• SGC
• SGCB

Background

The dystrophin-glycoprotein complex (DGC) is a multisubunit protein complex that spans the sarcolemma and provides structural linkage between the subsarcolemmal cytoskeleton and the extracellular matrix of muscle cells. There are 3 main subcomplexes of the DGC: the cytoplasmic proteins dystrophin (DMD; MIM 300377) and syntrophin (SNTA1; MIM 601017), the alpha- and beta-dystroglycans (see MIM 128239), and the sarcoglycans (see, e.g., SGCA; MIM 600119) (Crosbie et al., 2000 [PubMed 10942431]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SGCB