beta Amyloid 42 Peptide

Western Blot: beta Amyloid 42 Peptide [NBP3-18318] - Western blot of amyloid beta 1-42 monomers (NBP3-18318, left), oligomers (middle) and fibrils (right) using anti-amyloid beta 6E10 antibody. Amyloid beta constructs at 160 pmol were run on 4-12% Bis-Tris SDS-PAGE, transferred to nitrocellulose in the presence of 0.02% v/v Tween-20, and blotted with 1:1000 mouse 6E10 primary antibody (Biolegend). Oligomers observed under TEM/AFM show distinct dimer/trimer bands as well as a signal from ~37-75 kDa (middle). Fibrils observed under TEM/AFM show a signal greater than 100 kDa and a distinct signal in the stacking gel (right).

In vitro assay: beta Amyloid 42 Peptide [NBP3-18318] - Amyloid beta 1-42 oligomers and fibrils show a dose-dependent toxicity to primary rat cortical neurons, but not monomers (NBP3-18318). Survival of rat primary cortical neurons 14 days after treatment with different concentrations of (A) monomers, (B) oligomers or (C) fibrils quantified by MAP2 positive neurons and expressed as a percentage of control. Fibrils and respective vehicle controls were initially sonicated in a Bioruptor. Test conditions were run in the same plate as untreated control and vehicle controls, which consisted of buffer without amyloid beta 1-42 protein. Data expressed as mean +/- s.e.m. (n=6). A global analysis of the data was performed using a one-way ANOVA followed by Dunnetts test; ** p<0.01 stats vs control; ## p<0.01, #### p<0.0001 stats vs vehicle control.

Immunomicroscopy: beta Amyloid 42 Peptide [NBP3-18318] - AFM of amyloid beta 1-42 monomers (NBP3-18318, left), oligomers (middle) and fibrils (right). Atomic force microscopy analysis of 1.0 mg/mL samples diluted to 0.1 mg/mL in dH2O, mounted on freshly cleaved mica, washed, dried and analyzed with tapping mode. Representative images are 2.5 x 2.5 m x-y with a z-range of 10 nm.

Electron Microscopy: beta Amyloid 42 Peptide [NBP3-18318] - TEM of amyloid beta 1-42 monomers (NBP3-18318, left), oligomers (middle) and fibrils (right). Negative stain transmission electron microscopy images acquired at 80 Kv on carbon coated 400 mesh copper grids using phosphotungstic acid and uranyl acetate stain. Scale bar = 100 nm.

• Reactivity: Hu
• Applications: WB, Func, IHC, MS

beta Amyloid 42 Peptide Summary

• Description: A synthetic peptide treated with HFIP corresponding to human beta Amyloid 42.

  Amino Acid Sequence: DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA

• Localization: Cell Membrane|Intracellular Vesicles
• Protein/Peptide Type: Peptide
• Gene: APP
• Purity: >98%, by HPLC

Applications/Dilutions

• Dilutions:
  • Electron Microscopy
  • Immunomicroscopy
  • In vitro assay
  • In vivo assay
  • Western Blot
• Theoretical MW: 4.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -70C. Avoid freeze-thaw cycles.
• Buffer: Dry powder.
• Preservative: No Preservative
• Purity: >98%, by HPLC
• Reconstitution Instructions: See Reconstitution Instructions PDF for detailed protocol

Alternate Names for beta Amyloid 42 Peptide

• AAA
• Abeta
• ABPP
• AD1
• alpha-sAPP
• Amyloid-beta precursor protein
• APPI
• Beta-Amyloid Peptide(1-42)
• CTFgamma
• CVAP
• PN2
• PreA4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: APP