beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody

Western Blot: beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody [NBP2-26184] - Staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Discontinued

beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody Summary

• Immunogen: Peptide with sequence C-HVETPRNYKLRGD corresponding to internal region according to NP_061114.2.
• Specificity: Reported variants represent identical protein: NP_473366.1, NP_061114.2.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: B3GAT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 38 kDa band observed in human brain (frontal cortex) lysates (calculated MW of 38.3 kDa band according to NP_061114.2). An additional band of 17 kDa band was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than WB.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody

• B3GAT1
• beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
• Beta-1,3-glucuronyltransferase 1
• CD57
• EC 2.4.1.135
• galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
• GLCATP
• GlcAT-P
• GLCATPGLCUATP
• GLCUATP
• glcUAT-P
• Glucuronosyltransferase P
• HNK1
• LEU7
• NK1
• NK-1
• UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase

Background

CD57 represents a 100 kDa oligosaccharide antigenic determinant present on a variety of polypeptides, lipids and chondroitan sulfate proteoglycans. It is expressed on 7-35% of normal peripheral blood lymphocytes, including subsets of NK cells and CD8+ T lymphocytes. The function of CD57 is unknown. (1-6)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody (NBP2-26184)(1)

Publication using NBP2-26184

• Kahler AK, Djurovic S, Rimol LM et al. Candidate gene analysis of the human natural killer-1 carbohydrate pathway and perineuronal nets in schizophrenia: B3GAT2 is associated with disease risk and cortical surface area. Biol Psychiatry 2011-01-01 [PMID: 20950796]

Bioinformatics

• Gene Symbol: B3GAT1
• Entrez:
  • 27087(Human)
  • 76898(Mouse)
  • 117108(Rat)