beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody (1002707) [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: IHC
• Clone: 1002707
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 594

beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody (1002707) [Alexa Fluor® 594] Summary

• Additional Information: Human B3GAT1 MAb (Clone 1002707)
• Immunogen: Chinese Hamster Ovary cell line, CHO-derived human β‑1,3‑Glucuronyltransferase 1/B3GAT1
  His25-Ile334
  Accession # Q9P2W7
• Specificity: Detects human beta ‑1,3‑Glucuronyltransferase 1/B3GAT1 in direct ELISAs.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for beta-1,3-Glucuronyltransferase 1/B3GAT1 Antibody (1002707) [Alexa Fluor® 594]

• B3GAT1
• beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
• Beta-1,3-glucuronyltransferase 1
• CD57
• EC 2.4.1.135
• galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1
• GLCATP
• GlcAT-P
• GLCATPGLCUATP
• GLCUATP
• glcUAT-P
• Glucuronosyltransferase P
• HNK1
• LEU7
• NK1
• NK-1
• UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase

Background

B3GAT1 is a key enzyme involved in human natural killer1 (HNK1) epitope synthesis. It adds a glucuronic residue to the terminal lactosamine residue (Gal beta 14GlcNAc) of a glycoprotein or glycolipid, which can be further sulfated to become the HNK1 epitope, a unique trisaccharide structure, HSO₃-3GlcA beta 1-3Gal beta 1-4GlcNAc (1, 2). The enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol (3). The HNK1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules in addition to some glycolipids in the extracellular matrix and on the cell surface in the nervous system, where it is involved in cell-cell and cell-substratum interaction and recognition during the development of the nervous system (4). Like most known glycosyltransferases, B3GAT1 is a type II Golgi-resident transmembrane protein with a short N-terminal cytoplasmic domain and a single pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. The enzyme activity was assayed using a phosphatase-coupled method (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.