BDH2 Antibody

Western Blot: BDH2 Antibody [NB100-93379] - HEK293 overexpressing BDH2 and probed (mock transfection in first lane).

Immunohistochemistry-Paraffin: BDH2 Antibody [NB100-93379] - (5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: BDH2 Antibody [NB100-93379] - (0.3ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer).

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

BDH2 Antibody Summary

• Description: Novus Biologicals Goat BDH2 Antibody (NB100-93379) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-BDH2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-TKKKQIDQFANE corresponding to internal region according to NP_064524.3.
• Epitope: C-TKKKQIDQFANE
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BDH2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3-1 ug/ml
• Application Notes: WB: Approx. 26 kDa band observed in human brain (cerebellum), kidney and liver lysates (calculated MW of 26.7 kDa band according to NP_064524.3). In transfected HEK293 transiently expressing BDH2 a band of approx. 29 kDa is observed. This band is not observed in the non-transfected HEK293.
• Control:

  BDH2 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for BDH2 Antibody

• 3-hydroxybutyrate dehydrogenase, type 2
• dehydrogenase/reductase (SDR family) member 6
• Dehydrogenase/reductase SDR family member 6
• DHRS6
• EC 1.1.1.-
• EC 1.1.1.30
• EFA6R
• FLJ13261
• Oxidoreductase UCPA
• PRO20933
• R-beta-hydroxybutyrate dehydrogenase
• SDR15C1,3-hydroxybutyrate dehydrogenase type 2
• short chain dehydrogenase/reductase family 15C, member 1
• UCPA-OR
• UNQ6308

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BDH2 Antibody (NB100-93379)(1)

Publication using NB100-93379

• Guo K, Lukacik P, Papagrigoriou E et al. Characterization of human DHRS6, an orphan short chain dehydrogenase/reductase enzyme: a novel, cytosolic type 2 R-beta-hydroxybutyrate dehydrogenase. J Biol Chem 2006-04-14 [PMID: 16380372]

Bioinformatics

• Gene Symbol: BDH2
• Entrez:
  • 56898(Human)
  • 69772(Mouse)
  • 295458(Rat)
• Uniprot:
  • NP_064524.3(Human)