Bcl-6 Antibody (BCL6/1718) [Alexa Fluor™ Plus 594]

• Reactivity: Hu
• Applications: IHC, MA
• Clone: BCL6/1718
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 594

Bcl-6 Antibody (BCL6/1718) [Alexa Fluor™ Plus 594] Summary

• Immunogen: Recombinant human bcl-6 protein fragment (aa256-389) (exact sequence is proprietary) (Uniprot: P41182)
• Localization: Nuclear
• Marker: Follicular Lymphoma Marker
• Specificity: Recognizes a protein of 95kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large ('LH') cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: BCL6
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin
  • Protein Array
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Bcl-6 Antibody (BCL6/1718) [Alexa Fluor™ Plus 594]

• B-cell CLL/lymphoma 6
• B-cell lymphoma 6 protein transcript
• Bcl-5
• BCL5B-cell lymphoma 5 protein
• Bcl6
• Bcl-6
• BCL6A
• cys-his2 zinc finger transcription factor
• LAZ3
• LAZ3ZBTB27B-cell lymphoma 6 protein
• lymphoma-associated zinc finger gene on chromosome 3
• Protein LAZ-3
• ZBTB27
• Zinc finger and BTB domain-containing protein 27
• Zinc finger protein 51ZNF51
• zinc finger transcription factor BCL6S
• ZNF51

Background

Bcl6 reacts with Bcl6 gene product in follicular lymphomas, diffuse large B cell lymphomas, Burkitt's lymphomas and in nodular, lymphocyte predominant Hodgkin's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCL6