Bcl-6 Antibody (BCL6/1475) [Alexa Fluor® 532]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF
Clone
BCL6/1475
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 532

Order Details

Bcl-6 Antibody (BCL6/1475) [Alexa Fluor® 532] Summary

Immunogen
Recombinant human bcl-6 protein fragment (aa256-389)
Localization
Nuclear
Marker
Follicular Lymphoma Marker
Specificity
The specificity of this monoclonal antibody to human Bcl-6 was validated by HuProt(TM) Array, containing more than 19,000, full-length human proteins. Recognizes a protein of 95kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL). The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large (L&H) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
BCL6
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Bcl-6 Antibody (BCL6/1475) [Alexa Fluor® 532]

  • B-cell CLL/lymphoma 6
  • B-cell lymphoma 6 protein transcript
  • Bcl-5
  • BCL5B-cell lymphoma 5 protein
  • Bcl6
  • Bcl-6
  • BCL6A
  • cys-his2 zinc finger transcription factor
  • LAZ3
  • LAZ3ZBTB27B-cell lymphoma 6 protein
  • lymphoma-associated zinc finger gene on chromosome 3
  • Protein LAZ-3
  • ZBTB27
  • Zinc finger and BTB domain-containing protein 27
  • Zinc finger protein 51ZNF51
  • zinc finger transcription factor BCL6S
  • ZNF51

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, ICC/IF

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Bcl-6 Antibody (NBP2-59597AF532) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-59597AF350
Alexa Fluor 405 NBP2-59597AF405
Alexa Fluor 488 NBP2-59597AF488
Alexa Fluor 532 NBP2-59597AF532
Alexa Fluor 594 NBP2-59597AF594
Alexa Fluor 647 NBP2-59597AF647
Alexa Fluor 700 NBP2-59597AF700
Alexa Fluor 750 NBP2-59597AF750
Allophycocyanin NBP2-59597APC
Biotin NBP2-59597B
DyLight 350 NBP2-59597UV
DyLight 405 NBP2-59597V
DyLight 488 NBP2-59597G
DyLight 550 NBP2-59597R
DyLight 594 NBP2-59597DL594
DyLight 650 NBP2-59597C
DyLight 680 NBP2-59597FR
DyLight 755 NBP2-59597IR
FITC NBP2-59597F
HRP NBP2-59597H
Janelia Fluor 549 NBP2-59597JF549
Janelia Fluor 646 NBP2-59597JF646
PE NBP2-59597PE
PerCP NBP2-59597PCP

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Bioinformatics

Gene Symbol BCL6