Bcl-10 Antibody (SN74-04)

Western Blot: Bcl-10 Antibody (SN74-04) [NBP2-67714] - Western blot analysis of Bcl-10 on different cell lysates using anti-Bcl-10 antibody at 1/500 dilution. Positive control: Lane 1: HUVEC Lane 2:SW480

Immunocytochemistry/Immunofluorescence: Bcl-10 Antibody (SN74-04) [NBP2-67714] - Staining Bcl10 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Bcl-10 Antibody (SN74-04) [NBP2-67714] - Analysis of paraffin-embedded human tonsil tissue using anti-Bcl10 antibody. Counter stained with hematoxylin.

Western Blot: Bcl-10 Antibody (SN74-04) [NBP2-67714] - Analysis of Bcl10 on different cell lysates using anti-Bcl10 antibody at 1/500 dilution.Positive control: Lane 1: HUVEC Lane 2:SW480

Immunocytochemistry/Immunofluorescence: Bcl-10 Antibody (SN74-04) [NBP2-67714] - Staining Bcl10 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

Bcl-10 Antibody (SN74-04) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Bcl-10 aa 198-233 / 233. (SwissProt: O95999 Human)
• Localization: Cytoplasm, Membrane raft.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BCL10
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Bcl-10 Antibody (SN74-04)

• B-cell CLL/lymphoma 10CIPERCLAPB-cell lymphoma/leukemia 10
• Bcl10
• Bcl-10
• CARD-containing apoptotic signaling protein
• CARD-containing molecule enhancing NF-kappa-B
• CARD-containing proapoptotic protein
• CARD-like apoptotic protein
• CARMEN
• caspase-recruiting domain-containing protein
• cCARMEN
• c-E10
• c-E10bcl-10
• CED-3/ICH-1 prodomain homologous E10-like regulator
• Cellular homolog of vCARMEN
• cellular-E10
• CIPER
• CLAP
• hCLAP
• Mammalian CARD-containing adapter molecule E10
• mE10
• mE10CARD containing molecule enhancing NF-kB

Background

Bcl-10 is an adaptor protein with a caspase recruitment domain (CARD) that promotes apoptosis and activates pro-caspase 9 and NF-kB (1-2). Bcl-10 also interacts with other CARD domain containing proteins including CARD9, 10, 11 and 14. Bcl-10 is found to form a complex with MALT1, a protein known to be translocated in MALT lymphomas. Bcl-10 and MALT1 play a critical role in antigen receptor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCL10