Bcl-10 Antibody (4F8)


Western Blot: Bcl-10 Antibody (4F8E8H8) [NB100-2180] - Analysis using BCL10 mouse mAb against NIH/3T3 (1), Hela (2), MCF-7 (3) and Jurkat (4) cell lysate.
Immunocytochemistry/ Immunofluorescence: Bcl-10 Antibody (4F8E8H8) [NB100-2180] - Analysis of Hela cells using BCL10 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 ...read more
Immunohistochemistry-Paraffin: Bcl-10 Antibody (4F8E8H8) [NB100-2180] - Analysis of human breast carcinoma (A) and liver carcinoma (B), showing cytoplasmic localization using BCL10 mouse mAb with DAB staining.
Flow Cytometry: Bcl-10 Antibody (4F8E8H8) [NB100-2180] - Analysis of Hela cells using BCL10 mouse mAb (green) and negative control (purple).

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Bcl-10 Antibody (4F8) Summary

Purified recombinant fragment of human Bcl10 expressed in E. coli.
BCL-10 (4F8E8H8)
Protein A or G purified
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  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
Application Notes
IHC(P): Dilution 1: 100- 1: 500 IHC(F): Dilution 1: 200- 1: 1000 Western blot: Dilution 1: 2000- 1: 5000 ELISA: Propose dilution 1: 10000. Determining optimal working dilutions by titration test.
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.03% Sodium Azide
Protein A or G purified

Alternate Names for Bcl-10 Antibody (4F8)

  • B-cell CLL/lymphoma 10CIPERCLAPB-cell lymphoma/leukemia 10
  • Bcl10
  • Bcl-10
  • CARD-containing apoptotic signaling protein
  • CARD-containing molecule enhancing NF-kappa-B
  • CARD-containing proapoptotic protein
  • CARD-like apoptotic protein
  • caspase-recruiting domain-containing protein
  • c-E10
  • c-E10bcl-10
  • CED-3/ICH-1 prodomain homologous E10-like regulator
  • Cellular homolog of vCARMEN
  • cellular-E10
  • CLAP
  • hCLAP
  • Mammalian CARD-containing adapter molecule E10
  • mE10
  • mE10CARD containing molecule enhancing NF-kB


Bcl-10 is a 31kDa protein containing a caspase recruitment domain (CARD). It plays an important role in apoptosis and activating NF-kappaB. The research suggested that it interacted with other CARD domain containing proteins including CARD9, 10, 11 and 14, which were thought to function as upstream regulators in NF-kappaB signaling. Bcl-10 is found to form a complex with MALT1 which encoded by another gene known to be translocated in MALT lymphoma. MALT1 and Bcl-10 are thought to synergize in the activation of NF-kappaB, and the deregulation of either of them may contribute to the same pathogenetic process that leads to the malignancy.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Gene Symbol BCL10