BCAT2 Antibody

Immunohistochemistry-Paraffin: BCAT2 Antibody [NBP1-32652] - Paraffin-embedded Ca922 xenograft, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: BCAT2 Antibody [NBP1-32652] - Paraffin-embedded Ca922 xenograft, using antibody at 1:100 dilution.

Western Blot: BCAT2 Antibody [NBP1-32652] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BCAT2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

BCAT2 Antibody Summary

• Description: Novus Biologicals Rabbit BCAT2 Antibody (NBP1-32652) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human BCAT2. The exact sequence is proprietary.
• Localization: Isoform A: Mitochondrion, Isoform B: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BCAT2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (83%), Bovine (87%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for BCAT2 Antibody

• BCAM
• BCAT(m)
• BCAT2
• BCATM
• BCT2
• BCT2EC 2.6.1.42
• branched chain amino-acid transaminase 2, mitochondrial
• branched chain aminotransferase 2, mitochondrial
• branched-chain-amino-acid aminotransferase, mitochondrial
• Eca40
• Placental protein 18
• PP18

Background

The BCAT2 gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer thatcatalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multipletranscript variants enco

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BCAT2