BCAM/CD239 Antibody

Western blot shows lysates of mouse kidney tissue, mouse skeletal muscle tissue, rat heart tissue, and rat lung tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat BCAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8299) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for BCAM at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

BCAM was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse/Rat BCAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8299) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

BCAM/CD239 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse BCAM
  Met1-Ala541
  Accession # Q9R069
• Specificity: Detects mouse and rat BCAM in Western blots. In direct ELISAs, less than 50% cross-reactivity with recombinant human BCAM is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BCAM
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BCAM/CD239 Antibody

• antigen identified by monoclonal F8
• Auberger B antigen
• basal cell adhesion molecule (Lu and Au blood groups)
• basal cell adhesion molecule (Lutheran blood group)
• basal cell adhesion molecule
• B-CAM cell surface glycoprotein
• BCAM
• B-cell adhesion molecule
• CD239 antigen
• CD239
• F8/G253 antigen
• glycoprotein 95kDa
• LUAU
• Lutheran antigen
• Lutheran blood group (Auberger b antigen included)
• Lutheran blood group glycoprotein
• MSK19

Background

Mouse BCAM (Basal cell adhesion molecule, B-CAM cell surface glycoprotein,Lutheran antigen, and CD239) is a 70-90 kDa membrane glycoprotein. BCAM has wide tissue distribution and is expressed on erythrocytes, the endothelium of blood vessels and on the basal layer of cells in the epithelia. The expression of BCAM in normal tissues is higher in fetal versus adult tissues. BCAM expression is also upregulated in activated keratinocytes and following malignant transformation in some cell types in vivo and in vitro. BCAM has been shown to be an adhesion molecule that binds laminin, a basement membrane protein involved in cell differentiation, adhesion, migration and proliferation. Over aa 1-541, mouse BCAM is 91% identical to rat BCAM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BCAM/CD239 Antibody (AF8299)(4)

Publications using AF8299

• A Soffer, A Mahly, K Padmanabha, J Cohen, O Adir, E Loushi, Y Fuchs, SE Williams, C Luxenburg Apoptosis and tissue thinning contribute to symmetric cell division in the developing mouse epidermis in a nonautonomous way PloS Biology, 2022-08-15;20(8):e3001756. 2022-08-15 [PMID: 35969606] (IF, Western Blot, Mouse)
• C Xu, VV Dinh, K Kruse, HW Jeong, EC Watson, S Adams, F Berkenfeld, M Stehling, SJ Rasouli, R Fan, R Chen, I Bedzhov, Q Chen, K Kato, ME Pitulescu, RH Adams Induction of osteogenesis by bone-targeted Notch activation Elife, 2022-02-04;11(0):. 2022-02-04 [PMID: 35119364] (IHC, Mouse)
• SC Schüler, JM Kirkpatric, M Schmidt, D Santinha, P Koch, S Di Sanzo, E Cirri, M Hemberg, A Ori, J von Maltza Extensive remodeling of the extracellular matrix during aging contributes to age-dependent impairments of muscle stem cell functionality Cell Reports, 2021-06-08;35(10):109223. 2021-06-08 [PMID: 34107247] (IHC, Mouse)
• UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams Cell-matrix signals specify bone endothelial cells during developmental osteogenesis Nat. Cell Biol, 2017-02-20;19(3):189-201. 2017-02-20 [PMID: 28218908] (IHC, Mouse)

Bioinformatics

• Gene Symbol: BCAM
• Uniprot:
  • Q9R069()