BCAM/CD239 Antibody (87207)

Huh‑7 human hepatoma cell line was stained with Mouse Anti-Human BCAM Monoclonal Antibody (Catalog # MAB1481, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready, ELISA(Cap)
• Clone: 87207
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

BCAM/CD239 Antibody (87207) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human BCAM
  Glu32-Ala547
  Accession # CAA58449
• Specificity: Detects human BCAM in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) ALCAM, rhEpCAM, recombinant mouse (rm) MAdCAM-1, rhMCAM, rhNCAM-L1, rmOCAM, or rmTROP‑2 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: BCAM
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Human BCAM Biotinylated Antibody (Catalog number BAF148)
  Standard: Recombinant Human BCAM Fc Chimera (Catalog number 148-BC)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for BCAM/CD239 Antibody (87207)

• antigen identified by monoclonal F8
• Auberger B antigen
• basal cell adhesion molecule (Lu and Au blood groups)
• basal cell adhesion molecule (Lutheran blood group)
• basal cell adhesion molecule
• B-CAM cell surface glycoprotein
• BCAM
• B-cell adhesion molecule
• CD239 antigen
• CD239
• F8/G253 antigen
• glycoprotein 95kDa
• LUAU
• Lutheran antigen
• Lutheran blood group (Auberger b antigen included)
• Lutheran blood group glycoprotein
• MSK19

Background

Basal-Cell Adhesion Molecule (BCAM) and Lutheran blood group glycoprotein (LU) are two alternatively spliced variants of a single immunoglobulin superfamily (IgSF) protein that differ in the length of their cytoplasmic tails. BCAM cDNA encodes a 628 amino acid (aa) residues precursor protein with a putative 31 aa signal peptide, a 597 aa extracellular domain containing three C2 type and two V-type Ig-like domains, a 21 aa transmembrane domain, and a 19 aa cytoplasmic domain. Compared to the 40 aa cytoplasmic domain present in LU, the BCAM cytoplasmic tail lacks the putative Src homology 3 (SH3) binding site that may be involved in mediating intracellular signaling. BCAM/LU has wide tissue distribution and is expressed on erythrocytes, the endothelium of blood vessels and on the basal layer of cells in the epithelia. The expression of BCAM/LU in normal tissues is higher in fetal versus adult tissues. BCAM/LU expression is also upregulated in sickle cell disease red blood cells, in activated keratinocytes and following malignant transformation in some cell types in vivo and in vitro. BCAM/LU has been shown to be an adhesion molecule that binds laminin, a basement membrane protein involved in cell differentiation, adhesion, migration and proliferation.

1. Campbell, I.G. et al. (1994) Cancer Research 54:5761.
2. Parsons, S.F. et al. (1995) Proc. Natl., Acad. Sci. USA, 92:5496.
3. Udani, M. et al. (1998) 101:2550.
4. Schon, M. et al. (2000) J. Invest. Dermatol, 115:1047.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for BCAM/CD239 Antibody (MAB1481)(6)

Publications using MAB1481

• Suresh Sivakumar, Sonja Lieber, Damiano Librizzi, Corinna Keber, Leah Sommerfeld, Florian Finkernagel et al. Basal cell adhesion molecule promotes metastasis‐associated processes in ovarian cancer Clinical and Translational Medicine 1/16/2023 [PMID: 36647260]
• Y Enomoto-Ok, Y Maeda, N Harashima, Y Sugawara, F Katagiri, K Hozumi, KM Hui, M Nomizu, Y Ito, Y Kikkawa An Anti-Human Lutheran Glycoprotein Phage Antibody Inhibits Cell Migration on Laminin-511: Epitope Mapping of the Antibody PLoS ONE, 2017-01-06;12(1):e0167860. 2017-01-06 [PMID: 28060819] (Bioassay, Human)
• Zhenyu Cai, Anling Zhang, Swati Choksi, Weihua Li, Tao Li, Xue-Min Zhang et al. Activation of cell-surface proteases promotes necroptosis, inflammation and cell migration Cell Research 8/1/2016 [PMID: 27444869]
• Chaar V, Laurance S, Lapoumeroulie C, Cochet S, De Grandis M, Colin Y, Elion J, Le Van Kim C, El Nemer W Hydroxycarbamide decreases sickle reticulocyte adhesion to resting endothelium by inhibiting endothelial lutheran/basal cell adhesion molecule (Lu/BCAM) through phosphodiesterase 4A activation. J Biol Chem, 2014-03-10;289(16):11512-21. 2014-03-10 [PMID: 24616094] (Neutralization, Human)
• Kikkawa Y, Ogawa T, Sudo R, Yamada Y, Katagiri F, Hozumi K, Nomizu M, Miner J The lutheran/basal cell adhesion molecule promotes tumor cell migration by modulating integrin-mediated cell attachment to laminin-511 protein. J Biol Chem, 2013-09-13;288(43):30990-1001. 2013-09-13 [PMID: 24036115] (Western Blot, Human)
• Kikkawa Y, Miwa T, Tohara Y, Hamakubo T, Nomizu M An antibody to the lutheran glycoprotein (Lu) recognizing the LU4 blood type variant inhibits cell adhesion to laminin alpha5. PLoS ONE, 2011-08-12;6(8):e23329. 2011-08-12 [PMID: 21858073] (Blocking, Epitope Mapping, Human)

Bioinformatics

• Gene Symbol: BCAM
• Uniprot:
  • CAA58449()